Abstract

Dr. Sale proposes to define the mechanisms that regulate dynein activity by modulating motor function and controlling where the motor is localized.
Two specific aims are proposed: (1) to determine how changes in IC138 phosphorylation regulate the I1 dynein complex; and (2) to determine the mechanism by which I1 is targeted to the inner doublet microtubules, and to test the hypothesis that axonemal kinase and phosphatase are anchored.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM051173-15
Application #
2900818
Study Section
Cellular Biology and Physiology Subcommittee 1 (CBY)
Project Start
1985-07-01
Project End
2002-03-31
Budget Start
1999-04-01
Budget End
2000-03-31
Support Year
15
Fiscal Year
1999
Total Cost
Indirect Cost

  Institution

Name
Emory University
Department
Anatomy/Cell Biology
Type
Schools of Medicine
DUNS #
042250712
City
Atlanta
State
GA
Country
United States
Zip Code
30322

  Publications

King, Stephen M; Sale, Winfield S (2018) Fifty years of microtubule sliding in cilia. Mol Biol Cell 29:698-701
Hunter, Emily L; Lechtreck, Karl; Fu, Gang et al. (2018) The IDA3 adapter, required for intraflagellar transport of I1 dynein, is regulated by ciliary length. Mol Biol Cell 29:886-896
Yamamoto, Ryosuke; Obbineni, Jagan M; Alford, Lea M et al. (2017) Chlamydomonas DYX1C1/PF23 is essential for axonemal assembly and proper morphology of inner dynein arms. PLoS Genet 13:e1006996
Alford, Lea M; Stoddard, Daniel; Li, Jennifer H et al. (2016) The nexin link and B-tubule glutamylation maintain the alignment of outer doublets in the ciliary axoneme. Cytoskeleton (Hoboken) 73:331-40
Vasudevan, Krishna Kumar; Song, Kangkang; Alford, Lea M et al. (2015) FAP206 is a microtubule-docking adapter for ciliary radial spoke 2 and dynein c. Mol Biol Cell 26:696-710
Vasudevan, Krishna Kumar; Jiang, Yu-Yang; Lechtreck, Karl F et al. (2015) Kinesin-13 regulates the quantity and quality of tubulin inside cilia. Mol Biol Cell 26:478-94
Yang, Fan; Pavlik, Jacqueline; Fox, Laura et al. (2015) Alcohol-induced ciliary dysfunction targets the outer dynein arm. Am J Physiol Lung Cell Mol Physiol 308:L569-76
Avasthi, Prachee; Onishi, Masayuki; Karpiak, Joel et al. (2014) Actin is required for IFT regulation in Chlamydomonas reinhardtii. Curr Biol 24:2025-32
Viswanadha, Rasagnya; Hunter, Emily L; Yamamoto, Ryosuke et al. (2014) The ciliary inner dynein arm, I1 dynein, is assembled in the cytoplasm and transported by IFT before axonemal docking. Cytoskeleton (Hoboken) 71:573-86
Wirschell, Maureen; Olbrich, Heike; Werner, Claudius et al. (2013) The nexin-dynein regulatory complex subunit DRC1 is essential for motile cilia function in algae and humans. Nat Genet 45:262-8

Showing the most recent 10 out of 36 publications