Abstract

Dr. Matsudaira proposes to continue studies of the Limulus acrosome by: (1) solving the structure of the acrosomal filament and actin bundle and describing calcium-calmodulin-dependent changes in the conformation of actin and scruin; (2) identifying the binding sites involved in scruin-actin, scruin-calmodulin, and scruin-scruin interactions; and (3) measuring the energy stored in the true and false discharge and estimate the energy dissipated during the acrosome reaction.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
2R01GM052703-04
Application #
2634786
Study Section
Cellular Biology and Physiology Subcommittee 1 (CBY)
Project Start
1995-05-01
Project End
2002-04-30
Budget Start
1998-05-01
Budget End
1999-04-30
Support Year
4
Fiscal Year
1998
Total Cost
Indirect Cost

  Institution

Name
Whitehead Institute for Biomedical Research
Department
Type
DUNS #
076580745
City
Cambridge
State
MA
Country
United States
Zip Code
02142

  Publications

Shin, Jennifer H; Tam, Barney K; Brau, Ricardo R et al. (2007) Force of an actin spring. Biophys J 92:3729-33
Shin, J H; Gardel, M L; Mahadevan, L et al. (2004) Relating microstructure to rheology of a bundled and cross-linked F-actin network in vitro. Proc Natl Acad Sci U S A 101:9636-41
Gardel, M L; Shin, J H; MacKintosh, F C et al. (2004) Elastic behavior of cross-linked and bundled actin networks. Science 304:1301-5
Shin, Jennifer H; Mahadevan, L; So, P T et al. (2004) Bending stiffness of a crystalline actin bundle. J Mol Biol 337:255-61
Shin, Jennifer H; Mahadevan, L; Waller, Guillermina S et al. (2003) Stored elastic energy powers the 60-microm extension of the Limulus polyphemus sperm actin bundle. J Cell Biol 162:1183-8
Choe, S E; Li, L; Matsudaira, P T et al. (2000) Differential stabilization of two hydrophobic cores in the transition state of the villin 14T folding reaction. J Mol Biol 304:99-115
Mahadevan, L; Matsudaira, P (2000) Motility powered by supramolecular springs and ratchets. Science 288:95-100
Schmid, M F; Sherman, M B; Matsudaira, P et al. (1999) Scaling structure factor amplitudes in electron cryomicroscopy using X-Ray solution scattering. J Struct Biol 128:51-7
Sherman, M B; Jakana, J; Sun, S et al. (1999) The three-dimensional structure of the Limulus acrosomal process: a dynamic actin bundle. J Mol Biol 294:139-49
Choe, S E; Matsudaira, P T; Osterhout, J et al. (1998) Folding kinetics of villin 14T, a protein domain with a central beta-sheet and two hydrophobic cores. Biochemistry 37:14508-18

Showing the most recent 10 out of 13 publications