This application is focused on the analysis of mechanisms and functions of MT-based motors that the investigators postulate drive specific transport event that occurs during early development. They propose to : 1) characterize the structure, function and domain organization of a putative minus-end-directed kinesin holoenzyme and to analyze its role in nuclear transport and mitosis; 2) to further analyze structure-function relationships in the morphogenesis and function of mitotic spindles; 3) to biochemically characterize the vesicular cargo of the sea urchin embryonic mitotic spindle for delivery to different destinations and 4) to purify the Drosophila Nod chromosomal kinesin-like motor from transgenic fly lines, in order to analyze the mechanism by which it transports chromosomes along MTs.
| Scholey, Jonathan M; Civelekoglu-Scholey, Gul; Brust-Mascher, Ingrid (2016) Anaphase B. Biology (Basel) 5: |
| Brust-Mascher, Ingrid; Civelekoglu-Scholey, Gul; Scholey, Jonathan M (2015) Mechanism for Anaphase B: Evaluation of ""Slide-and-Cluster"" versus ""Slide-and-Flux-or-Elongate"" Models. Biophys J 108:2007-18 |
| Scholey, Jessica E; Nithianantham, Stanley; Scholey, Jonathan M et al. (2014) Structural basis for the assembly of the mitotic motor Kinesin-5 into bipolar tetramers. Elife 3:e02217 |
| Wang, Haifeng; Brust-Mascher, Ingrid; Civelekoglu-Scholey, Gul et al. (2013) Patronin mediates a switch from kinesin-13-dependent poleward flux to anaphase B spindle elongation. J Cell Biol 203:35-46 |
| Scholey, Jonathan M (2013) Compare and contrast the reaction coordinate diagrams for chemical reactions and cytoskeletal force generators. Mol Biol Cell 24:433-9 |
| Acar, Seyda; Carlson, David B; Budamagunta, Madhu S et al. (2013) The bipolar assembly domain of the mitotic motor kinesin-5. Nat Commun 4:1343 |
| de Lartigue, Jane; Brust-Mascher, Ingrid; Scholey, Jonathan M (2011) Anaphase B spindle dynamics in Drosophila S2 cells: Comparison with embryo spindles. Cell Div 6:8 |
| Tao, Li; Scholey, Jonathan M (2010) Purification and assay of mitotic motors. Methods 51:233-41 |
| Wang, Haifeng; Brust-Mascher, Ingrid; Cheerambathur, Dhanya et al. (2010) Coupling between microtubule sliding, plus-end growth and spindle length revealed by kinesin-8 depletion. Cytoskeleton (Hoboken) 67:715-28 |
| Civelekoglu-Scholey, Gul; Tao, Li; Brust-Mascher, Ingrid et al. (2010) Prometaphase spindle maintenance by an antagonistic motor-dependent force balance made robust by a disassembling lamin-B envelope. J Cell Biol 188:49-68 |
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