Abstract

The general hypothesis to be tested is that phosphorylation of connexin43 and/or Cx45 regulates a variety of cellular functions. The experiments will address whether: (1) phosphorylation of Cx43 and Cx45 regulates the kinetics of connexin trafficking and/or oligomerization into connexons; (2) connexin phosphorylation regulates the intra- and intermembrane interactions that lead to gap junction channel formation; and (3) phosphorylation of Cx43 and Cx45 regulates gap junction degradation.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
1R01GM055632-01
Application #
2024186
Study Section
Cellular Biology and Physiology Subcommittee 1 (CBY)
Project Start
1997-05-01
Project End
2001-04-30
Budget Start
1997-05-01
Budget End
1998-04-30
Support Year
1
Fiscal Year
1997
Total Cost
Indirect Cost

  Institution

Name
Fred Hutchinson Cancer Research Center
Department
Type
DUNS #
075524595
City
Seattle
State
WA
Country
United States
Zip Code
98109

  Publications

Slavi, Nefeli; Toychiev, Abduqodir H; Kosmidis, Stylianos et al. (2018) Suppression of connexin 43 phosphorylation promotes astrocyte survival and vascular regeneration in proliferative retinopathy. Proc Natl Acad Sci U S A 115:E5934-E5943
Solan, Joell L; Lampe, Paul D (2018) Spatio-temporal regulation of connexin43 phosphorylation and gap junction dynamics. Biochim Biophys Acta Biomembr 1860:83-90
Kam, Chen Yuan; Dubash, Adi D; Magistrati, Elisa et al. (2018) Desmoplakin maintains gap junctions by inhibiting Ras/MAPK and lysosomal degradation of connexin-43. J Cell Biol 217:3219-3235
Laird, Dale W; Lampe, Paul D (2018) Therapeutic strategies targeting connexins. Nat Rev Drug Discov :
Laird, Dale W; Naus, Christian C; Lampe, Paul D (2017) SnapShot: Connexins and Disease. Cell 170:1260-1260.e1
Aasen, Trond; Mesnil, Marc; Naus, Christian C et al. (2017) Gap junctions and cancer: communicating for 50 years. Nat Rev Cancer 17:74
Leybaert, Luc; Lampe, Paul D; Dhein, Stefan et al. (2017) Connexins in Cardiovascular and Neurovascular Health and Disease: Pharmacological Implications. Pharmacol Rev 69:396-478
Jacobsen, Nicole L; Pontifex, Tasha K; Li, Hanjun et al. (2017) Regulation of Cx37 channel and growth-suppressive properties by phosphorylation. J Cell Sci 130:3308-3321
Crassous, Pierre-Antoine; Shu, Ping; Huang, Can et al. (2017) Newly Identified NO-Sensor Guanylyl Cyclase/Connexin 43 Association Is Involved in Cardiac Electrical Function. J Am Heart Assoc 6:
Egbert, Jeremy R; Uliasz, Tracy F; Shuhaibar, Leia C et al. (2016) Luteinizing Hormone Causes Phosphorylation and Activation of the cGMP Phosphodiesterase PDE5 in Rat Ovarian Follicles, Contributing, Together with PDE1 Activity, to the Resumption of Meiosis. Biol Reprod 94:110

Showing the most recent 10 out of 79 publications