Receptor kinases (RKs) play an important role in the mechanisms witch which organisms perceive and respond to environmental signals; regulating growth and development in both animals and plants. The long-term goal of the proposed project is to elicit the signal transaction cascade regulated by RKs. This information will enhance our understanding of cellular transformation and carcinogenesis in animals and lead to the development of environmentally safe strategies for disease control in plants. The short-term goal of the proposed project is to establish the causal connection between pathogen induced activation of the rice receptor-like kinase, XA21, and the downstream defense responses. Based on information from receptor tyrosine kinases in animal systems, we hypothesize that the catalytic domain of XA21 plays a key role in XA21-mediated signal transduction and that specific residues as binding sites for intracellular proteins. These proteins may then directly or indirectly transduce various defense responses. We also hypothesize that the pathogen may secrete factors into the host cell that influence the signal transduction pathway. In partial support of these hypotheses, we have found that the intracellular domain can autophosphorylate itself in vitro, that a pathogen GTP-binding protein and a rice catalase interact specifically with the XA21 in plants undergoing a resistance response. To test these hypotheses, we propose to carry out the following objectives: 1. Confirm the essential role of phosphorylation in XA21-mediated disease resistance. 2. Identify and characterize rice proteins that interact with the Xa21- kinase. 3. Characterize the defense response and defense-related gene expression in the XA21-mediated defense pathway. 4. Determine the biological significance of the xoo/GBP interaction. Accomplishments of the proposed objectives will, for the first time, lead to the establishment of the causal connection between a plant receptor kinase and the associated downstream response.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM055962-04
Application #
6490119
Study Section
Biological Sciences 2 (BIOL)
Program Officer
Anderson, James J
Project Start
1999-01-01
Project End
2004-05-31
Budget Start
2002-01-01
Budget End
2004-05-31
Support Year
4
Fiscal Year
2002
Total Cost
$183,650
Indirect Cost
Name
University of California Davis
Department
Other Basic Sciences
Type
Schools of Earth Sciences/Natur
DUNS #
094878337
City
Davis
State
CA
Country
United States
Zip Code
95618
De Vleesschauwer, David; Filipe, Osvaldo; Hoffman, Gena et al. (2018) Target of rapamycin signaling orchestrates growth-defense trade-offs in plants. New Phytol 217:305-319
Harkenrider, Mitch; Sharma, Rita; De Vleesschauwer, David et al. (2016) Overexpression of Rice Wall-Associated Kinase 25 (OsWAK25) Alters Resistance to Bacterial and Fungal Pathogens. PLoS One 11:e0147310
Schwessinger, Benjamin; Li, Xiang; Ellinghaus, Thomas L et al. (2016) A second-generation expression system for tyrosine-sulfated proteins and its application in crop protection. Integr Biol (Camb) 8:542-5
Pruitt, Rory N; Schwessinger, Benjamin; Joe, Anna et al. (2015) The rice immune receptor XA21 recognizes a tyrosine-sulfated protein from a Gram-negative bacterium. Sci Adv 1:e1500245
Zuo, Shimin; Zhou, Xiaogang; Chen, Mawsheng et al. (2014) OsSERK1 regulates rice development but not immunity to Xanthomonas oryzae pv. oryzae or Magnaporthe oryzae. J Integr Plant Biol 56:1179-1192
Tripathi, Jaindra N; Lorenzen, Jim; Bahar, Ofir et al. (2014) Transgenic expression of the rice Xa21 pattern-recognition receptor in banana (Musa sp.) confers resistance to Xanthomonas campestris pv. musacearum. Plant Biotechnol J 12:663-73
Ronald, Pamela C (2014) Lab to farm: applying research on plant genetics and genomics to crop improvement. PLoS Biol 12:e1001878
McAndrew, Ryan; Pruitt, Rory N; Kamita, Shizuo G et al. (2014) Structure of the OsSERK2 leucine-rich repeat extracellular domain. Acta Crystallogr D Biol Crystallogr 70:3080-6
Park, Chang-Jin; Sharma, Rita; Lefebvre, Benoit et al. (2013) The endoplasmic reticulum-quality control component SDF2 is essential for XA21-mediated immunity in rice. Plant Sci 210:53-60
Han, Sang-Wook; Lee, Sang-Won; Bahar, Ofir et al. (2012) Tyrosine sulfation in a Gram-negative bacterium. Nat Commun 3:1153

Showing the most recent 10 out of 28 publications