How organisms respond to light and how photosensory receptors mediate light responses are some of the most basic questions in biology that affect human health. Cryptochromes (CRY) are blue/UV-A light receptors found in bacteria, plants, and animals including human, but the molecular mechanisms of CRYs remain not well understood. My laboratory uses Arabidopsis as the model system to study signaling mechanisms underlying CRY1 and CRY2 regulation of plant development. We recently identified, for the first time, blue light-dependent CRY-interacting proteins in plants. We propose to continue study CRY1 and CRY2 signaling mechanisms with three specific aims. First, we propose to investigate how CRY-interacting proteins regulate transcription, mRNA export, and protein stability. Second, we plan to investigate CRY regulation of cell-type- specific gene expression changes. Third, we propose to identify and characterize genes affecting blue light-induced CRY2 degradation and blue light- suppressed CIB1 degradation.

Public Health Relevance

Cryptochromes (CRY) are blue/UV-A light receptors regulating plant development and human health. We propose to study CRY-interacting proteins and how they mediate CRY regulation of gene expression in response to light.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
3R01GM056265-13S1
Application #
7921233
Study Section
Cellular Signaling and Regulatory Systems Study Section (CSRS)
Project Start
2009-09-30
Project End
2010-08-31
Budget Start
2009-09-30
Budget End
2010-08-31
Support Year
13
Fiscal Year
2009
Total Cost
$172,523
Indirect Cost
Name
University of California Los Angeles
Department
Biochemistry
Type
Schools of Arts and Sciences
DUNS #
092530369
City
Los Angeles
State
CA
Country
United States
Zip Code
90095
Wang, Qin; Zuo, Zecheng; Wang, Xu et al. (2018) Beyond the photocycle-how cryptochromes regulate photoresponses in plants? Curr Opin Plant Biol 45:120-126
Liu, Qing; Wang, Qin; Deng, Weixian et al. (2017) Molecular basis for blue light-dependent phosphorylation of Arabidopsis cryptochrome 2. Nat Commun 8:15234
Yang, Zhaohe; Liu, Bobin; Su, Jun et al. (2017) Cryptochromes Orchestrate Transcription Regulation of Diverse Blue Light Responses in Plants. Photochem Photobiol 93:112-127
Wang, Qin; Barshop, William D; Bian, Mingdi et al. (2017) The Blue Light-Dependent Phosphorylation of the CCE Domain Determines the Photosensitivity of Arabidopsis CRY2. Mol Plant 10:357
Liu, Qing; Wang, Qin; Liu, Bin et al. (2016) The Blue Light-Dependent Polyubiquitination and Degradation of Arabidopsis Cryptochrome2 Requires Multiple E3 Ubiquitin Ligases. Plant Cell Physiol 57:2175-2186
Liu, Bobin; Yang, Zhaohe; Gomez, Adam et al. (2016) Signaling mechanisms of plant cryptochromes in Arabidopsis thaliana. J Plant Res 129:137-48
Wang, Qin; Zuo, Zecheng; Wang, Xu et al. (2016) Photoactivation and inactivation of Arabidopsis cryptochrome 2. Science 354:343-347
Gao, Jie; Wang, Xu; Zhang, Meng et al. (2015) Trp triad-dependent rapid photoreduction is not required for the function of Arabidopsis CRY1. Proc Natl Acad Sci U S A 112:9135-40
Wang, Qin; Barshop, William D; Bian, Mingdi et al. (2015) The blue light-dependent phosphorylation of the CCE domain determines the photosensitivity of Arabidopsis CRY2. Mol Plant 8:631-43
Wang, Xu; Wang, Qin; Nguyen, Paula et al. (2014) Cryptochrome-mediated light responses in plants. Enzymes 35:167-89

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