The long-term goal is to understand how the centrosome assembles and functions in animal cells. The centrosome is the major microtubule nucleating and organizing center that participates in organizing interphase cytoplasm and mitotic spindle poles. In mitosis, a bipolar spindle is organized efficiently from two centrosomes duplicated during S phase of the cell cycle. Recent studies suggested that mis-regulated centrosome duplication lead to mono- or multi-polar spindles and chromosome mis-segregation, linking cancer formation to centrosome dysfunction. Therefore, understanding the structure and function of the centrosome is relevant to human health. This grant seeks to study the mechanism of centrosome-mediated microtubule nucleation and centrosome assembly via studying a large protein complex, the gamma-tubulin ring complex (gammaTuRC) found at the centrosome. gammaTuRC is an important microtubule nucleator at the centrosome and it is required for centrosome assembly. In this granting period, we hope to understand 1) how gammaTuRC is assembled from multiple subunits, 2) how gammaTuRC functions in microtubule nucleation and organization, 3) how gammaTuRC is recruited to the centrosome to participate in microtubule nucleation and centrosome assembly.
| Huang, Yuejia; Li, Teng; Ems-McClung, Stephanie C et al. (2018) Aurora A activation in mitosis promoted by BuGZ. J Cell Biol 217:107-116 |
| Zheng, Xiaobin; Hu, Jiabiao; Yue, Sibiao et al. (2018) Lamins Organize the Global Three-Dimensional Genome from the Nuclear Periphery. Mol Cell 71:802-815.e7 |
| Gigante, Crystal M; Dibattista, Michele; Dong, Frederick N et al. (2017) Lamin B1 is required for mature neuron-specific gene expression during olfactory sensory neuron differentiation. Nat Commun 8:15098 |
| Chen, Haiyang; Zheng, Xiaobin; Xiao, Danqing et al. (2016) Age-associated de-repression of retrotransposons in the Drosophila fat body, its potential cause and consequence. Aging Cell 15:542-52 |
| Tran, Joseph R; Zheng, Xiaobin; Zheng, Yixian (2016) Lamin-B1 contributes to the proper timing of epicardial cell migration and function during embryonic heart development. Mol Biol Cell 27:3956-3963 |
| Tran, Joseph R; Chen, Haiyang; Zheng, Xiaobin et al. (2016) Lamin in inflammation and aging. Curr Opin Cell Biol 40:124-130 |
| Zheng, Xiaobin; Yue, Sibiao; Chen, Haiyang et al. (2015) Low-Cell-Number Epigenome Profiling Aids the Study of Lens Aging and Hematopoiesis. Cell Rep 13:1505-1518 |
| Guo, Yuxuan; Zheng, Yixian (2015) Lamins position the nuclear pores and centrosomes by modulating dynein. Mol Biol Cell 26:3379-89 |
| Zheng, Xiaobin; Kim, Youngjo; Zheng, Yixian (2015) Identification of lamin B-regulated chromatin regions based on chromatin landscapes. Mol Biol Cell 26:2685-97 |
| Oakley, Berl R; Paolillo, Vitoria; Zheng, Yixian (2015) ?-Tubulin complexes in microtubule nucleation and beyond. Mol Biol Cell 26:2957-62 |
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