Aim 1 is to further define how XKCM1 destabilizes microtubules by a series of domain studies. A second series of studies will be focused on establishing evolutionary conservation of the microtubule destabilizing activity of other KinI family members.
Aim 2 is to identify and characterize proteins that interact with XKCM1. Here the applicant is particularly interested in identifying potential kinetochore components involved in association of XKCM1 with chromosomes.
Aim 3 is to investigate the mechanism of chromosome segregation in Xenopus extracts.
Aim 4 is to use reagents, developed in previous studies, to disrupt XKCMP1 function in vivo.
Aim 5 is to assess chromosome movement in cells where XKCMP1 function has been manipulated as in the previous aim.
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| Bisson-Filho, Alexandre W; Hsu, Yen-Pang; Squyres, Georgia R et al. (2017) Treadmilling by FtsZ filaments drives peptidoglycan synthesis and bacterial cell division. Science 355:739-743 |
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| Walczak, Claire E; Zong, Hailing; Jain, Sachin et al. (2016) Spatial regulation of astral microtubule dynamics by Kif18B in PtK cells. Mol Biol Cell 27:3021-3030 |
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