The extracellular matrix of fungal cells is referred to as the cell wall. The cell wall is composed of interlocked proteins and polysaccharides and serves as a buffer between the cell and its environment. In pathogenic fungi, the wall is critical for survival in the host. Enzymes involved in the regulation and assembly of the cell wall are therefore excellent targets for antifungal drugs. The spore wall of Saccharomyces cerevisiae is a complex structure that forms de novo during the process of sporulation. Assembly of the spore wall provides an excellent system to investigate both the process of fungal wall assembly and the signaling pathways that coordinate that process. The Gip1-Glc7 phosphatase complex mediates a signaling pathway that initiates assembly of the wall only after the completion of cytokinesis. A structure-function analysis of the Gip1 protein will be used to explore the basis by which membrane closure triggers Gip1-Glc7 signaling. The mechanism by which Gip1-Glc7 regulate a downstream component in the pathway, the transcription factor Gis1, will also be elucidated. Though the constituents of the fungal cell wall are known, the enzymes that assemble these materials into a functional wall are little understood. We have identified several genes encoding candidate enzymes involved in construction of the spore wall. These phenotypes of mutants in these genes will be characterized in detail. Additionally, the enzymatic activities of these proteins will be examined using an in vitro assembly assay. Components of the spore wall in S. cerevisiae are also found in the cell wall of the pathogen C. albicans where they are important for virulence of the organism. We will examine if these components are assembled into the C. albicans cell wall a similar fashion as in the S. cerevisiae spore wall. The remarkable ability of the spore wall to protect the spore from an array of environmental insults is due primarily to the outer layers of spore wall. These layers have two major constituents, chitosan, a glucosamine polysaccharide, and a unique polymer containing the crosslinked amino acid dityrosine. This dityrosine polymer is found on the outermost surface of the spore wall and acts in part as a diffusion barrier that limits the movement of soluble molecules into and out of the wall. To better understand how this polymer functions the structure of the dityrosine polymer will be determined using a combination of mass spectrometry and solid state 13C NMR.

Public Health Relevance

Fungal cell walls act as a barrier between the fungal cell and its environment. The wall is essential for a fungal pathogen to evade the host immune response and antifungal drugs commonly act by interfering with wall synthesis. This study focuses on the assembly of the spore wall of baker's yeast, Saccharomyces cerevisiae, as a model for fungal wall assembly. Experiments are targeted towards understanding the regulation, assembly pathways, and structure of wall components.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM072540-07
Application #
8328656
Study Section
Nuclear and Cytoplasmic Structure/Function and Dynamics Study Section (NCSD)
Program Officer
Nie, Zhongzhen
Project Start
2005-09-12
Project End
2014-08-31
Budget Start
2012-09-01
Budget End
2013-08-31
Support Year
7
Fiscal Year
2012
Total Cost
$320,523
Indirect Cost
$115,193
Name
State University New York Stony Brook
Department
Biochemistry
Type
Schools of Arts and Sciences
DUNS #
804878247
City
Stony Brook
State
NY
Country
United States
Zip Code
11794
Omerza, Gregory; Tio, Chong Wai; Philips, Timothy et al. (2018) The meiosis-specific Cdc20 family-member Ama1 promotes binding of the Ssp2 activator to the Smk1 MAP kinase. Mol Biol Cell 29:66-74
Nakamura, Tsuyoshi S; Numajiri, Yumi; Okumura, Yuuya et al. (2017) Dynamic localization of a yeast development-specific PP1 complex during prospore membrane formation is dependent on multiple localization signals and complex formation. Mol Biol Cell 28:3881-3895
Pappas, Samuel S; Bonifacino, Juan; Danek, Adrian et al. (2017) Eighth International Chorea-Acanthocytosis Symposium: Summary of Workshop Discussion and Action Points. Tremor Other Hyperkinet Mov (N Y) 7:428
Hoffmann, Reuben; Grabi?ska, Kariona; Guan, Ziqiang et al. (2017) Long-Chain Polyprenols Promote Spore Wall Formation in Saccharomyces cerevisiae. Genetics 207:1371-1386
Jin, Liang; Zhang, Kai; Sternglanz, Rolf et al. (2017) Predicted RNA Binding Proteins Pes4 and Mip6 Regulate mRNA Levels, Translation, and Localization during Sporulation in Budding Yeast. Mol Cell Biol 37:
Zhang, Kai; Needleman, Leor; Zhou, Sai et al. (2017) A Novel Assay Reveals a Maturation Process during Ascospore Wall Formation. J Fungi (Basel) 3:
Zhou, Sai; Sternglanz, Rolf; Neiman, Aaron M (2017) Developmentally regulated internal transcription initiation during meiosis in budding yeast. PLoS One 12:e0188001
Bemena, Leo D; Mukama, Omar; Neiman, Aaron M et al. (2017) In vitro reconstitution of the yeast spore wall dityrosine layer discloses the mechanism of its assembly. J Biol Chem 292:15880-15891
Okada, Hiroki; Kono, Keiko; Neiman, Aaron M et al. (2016) Examination and Disruption of the Yeast Cell Wall. Cold Spring Harb Protoc 2016:pdb.top078659
Okada, Hiroki; Neiman, Aaron M; Ohya, Yoshikazu (2016) Assay for Spore Wall Integrity Using a Yeast Predator. Cold Spring Harb Protoc 2016:pdb.prot085258

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