Metalloproteins carry out many cellular functions that are central to biology and human health. While our knowledge of how metalloproteins function has grown immensely thanks to technological advances, we still possess only a superficial understanding of the interplay between protein structure/dynamics and metal coordination/reactivity. As a result, it has not yet been possible a) to predict the functional mechanism of metalloproteins simply by looking at their structures, b) to emulate or improve upon the structures and functions of metalloproteins by de novo design, and c) to understand how complex bioinorganic functions may have emerged on simple peptide/protein scaffolds during natural evolution. The overarching goal of the proposed research program is to address these three challenges by designing and constructing protein scaffolds with increasingly more complex metal-based functions from scratch. Inspired by a hypothetical pathway for the natural evolution of metalloproteins, we have recently developed new approaches to metalloprotein design in which monomeric proteins are templated by metal ions to form novel supramolecular assemblies. The interfaces of these evolutionarily nave complexes are then engineered and evolved to create self-standing protein architectures with complex metal-based functions. In the proposed research, we will further develop these ?metal-templated protein design? strategies by capitalizing on two new protein scaffolds developed in our lab (DiCyt and TriCyt), which provide easy access to diverse metal coordination geometries, secondary-sphere environments and global properties (tunable structures, oligomeric states, flexibility/rigidity) that are difficult to attain with other protein design strategies. We will use DiCyt and TriCyt scaffolds to build metalloprotein assemblies for stable and selective coordination of first-row transition metal ions (Specific Aim 1), for challenging ester, amide and phospho-ester bond hydrolysis reactions (Specific Aim 2), and for redox catalytic reactions involving dioxygen binding and activation (Specific Aim 3). These efforts will not only uncover fundamental structure-function relationships that govern diverse metalloprotein activities, but also lead to better understanding of how bioinorganic complexity emerges in simple protein scaffolds.
Metalloproteins carry out diverse cellular functions (e.g., respiration, natural product synthesis, gene transcription, neurotransmission, cellular defense) that are central to biology and human health. By developing new strategies for the design of metalloproteins, the proposed research will test and uncover fundamental structure-function relationships that govern diverse metalloprotein activities and lead to better understanding of how bioinorganic complexity emerges in protein scaffolds. The resulting knowledge will have a positive impact on our fundamental knowledge of biological chemistry of metal ions and will lead to novel metalloproteins with potentially beneficial uses (e.g., for effective metal sequestration, bio-orthogonal catalytic reactions).
