Abstract

The objective of this proposal is to determine the role of calcium ions in the mechanism of action of gonadotropin releasing hormone (GnRH) stimulated release of luteinizing hormone (LH) from the pituitary. This objective will be obtained by analysis and manipulation of calcium movement, localization of calcium binding and contractile proteins, blockade and activation of calcium target proteins, direct measurement of cellular calcium potentials, and determination of specific sites of calcium action in response to stimulation by GnRN in pituitary cell gonadotropes.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
Eunice Kennedy Shriver National Institute of Child Health & Human Development (NICHD)
Type
Research Project (R01)
Project #
5R01HD019899-03
Application #
3317512
Study Section
Biochemical Endocrinology Study Section (BCE)
Project Start
1984-04-01
Project End
1987-07-31
Budget Start
1985-08-01
Budget End
1986-07-31
Support Year
3
Fiscal Year
1985
Total Cost
Indirect Cost

  Institution

Name
University of Iowa
Department
Type
Schools of Medicine
DUNS #
041294109
City
Iowa City
State
IA
Country
United States
Zip Code
52242

  Publications

Zarinan, Teresa; Perez-Solis, Marco A; Maya-Nunez, Guadalupe et al. (2010) Dominant negative effects of human follicle-stimulating hormone receptor expression-deficient mutants on wild-type receptor cell surface expression. Rescue of oligomerization-dependent defective receptor expression by using cognate decoys. Mol Cell Endocrinol 321:112-22
Janovick, Jo Ann; Conn, P Michael (2010) Use of pharmacoperones to reveal GPCR structural changes associated with constitutive activation and trafficking. Methods Enzymol 485:277-92
Janovick, Jo Ann; Conn, P Michael (2010) Salt bridge integrates GPCR activation with protein trafficking. Proc Natl Acad Sci U S A 107:4454-8
Ayala Yanez, Rodrigo; Conn, P Michael (2010) Protein disulfide isomerase chaperone ERP-57 decreases plasma membrane expression of the human GnRH receptor. Cell Biochem Funct 28:66-73
Conn, P Michael; Ulloa-Aguirre, Alfredo (2010) Trafficking of G-protein-coupled receptors to the plasma membrane: insights for pharmacoperone drugs. Trends Endocrinol Metab 21:190-7
Re, Michelle; Pampillo, Macarena; Savard, Martin et al. (2010) The human gonadotropin releasing hormone type I receptor is a functional intracellular GPCR expressed on the nuclear membrane. PLoS One 5:e11489
Jardón-Valadez, Eduardo; Aguilar-Rojas, Arturo; Maya-Núñez, Guadalupe et al. (2009) Conformational effects of Lys191 in the human GnRH receptor: mutagenesis and molecular dynamics simulations studies. J Endocrinol 201:297-307
Conn, P Michael; Janovick, Jo Ann (2009) Trafficking and quality control of the gonadotropin releasing hormone receptor in health and disease. Mol Cell Endocrinol 299:137-45
Lucca-Junior, W; Janovick, J A; Conn, P M (2009) Participation of the endoplasmic reticulum protein chaperone thio-oxidoreductase in gonadotropin-releasing hormone receptor expression at the plasma membrane. Braz J Med Biol Res 42:164-7
Ulloa-Aguirre, Alfredo; Conn, P Michael (2009) Targeting of G protein-coupled receptors to the plasma membrane in health and disease. Front Biosci (Landmark Ed) 14:973-94

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