Funding Agency

Agency
National Institute of Health (NIH)
Institute
Eunice Kennedy Shriver National Institute of Child Health & Human Development (NICHD)
Type
Research Project (R01)
Project #
5R01HD020055-10
Application #
2197954
Study Section
Biochemical Endocrinology Study Section (BCE)
Project Start
1985-08-01
Project End
1998-11-30
Budget Start
1995-12-01
Budget End
1996-11-30
Support Year
10
Fiscal Year
1996
Total Cost
Indirect Cost

  Institution

Name
Washington University
Department
Obstetrics & Gynecology
Type
Schools of Medicine
DUNS #
062761671
City
Saint Louis
State
MO
Country
United States
Zip Code
63130

  Publications

Thomas, James L; Duax, William L; Addlagatta, Anthony et al. (2004) Structure/function aspects of human 3beta-hydroxysteroid dehydrogenase. Mol Cell Endocrinol 215:73-82
Thomas, James L; Umland, Timothy C; Scaccia, Launa A et al. (2004) The higher affinity of human type 1 3beta-hydroxysteroid dehydrogenase (3beta-HSD1) for substrate and inhibitor steroids relative to human 3beta-HSD2 is validated in MCF-7 tumor cells and related to subunit interactions. Endocr Res 30:935-41
Thomas, James L; Duax, William L; Addlagatta, Anthony et al. (2003) Structure/function relationships responsible for coenzyme specificity and the isomerase activity of human type 1 3 beta-hydroxysteroid dehydrogenase/isomerase. J Biol Chem 278:35483-90
Thomas, James L; Mason, J Ian; Brandt, Stacey et al. (2002) Structure/function relationships responsible for the kinetic differences between human type 1 and type 2 3beta-hydroxysteroid dehydrogenase and for the catalysis of the type 1 activity. J Biol Chem 277:42795-801
Thomas, James L; Mason, J Ian; Brandt, Stacey et al. (2002) Differences in substrate and inhibitor kinetics of human type 1 and type 2 3beta-hydroxysteroid dehydrogenase are explained by the type 1 mutant, H156Y. Endocr Res 28:471-5
Thomas, J L; Mason, J I; Blanco, G et al. (2001) The engineered, cytosolic form of human type I 3beta-hydroxysteroid dehydrogenase/isomerase: purification, characterization and crystallization. J Mol Endocrinol 27:77-83
Thomas, J L; Evans, B W; Blanco, G et al. (1998) Site-directed mutagenesis identifies amino acid residues associated with the dehydrogenase and isomerase activities of human type I (placental) 3beta-hydroxysteroid dehydrogenase/isomerase. J Steroid Biochem Mol Biol 66:327-34
Mason, J I; Naville, D; Evans, B W et al. (1998) Functional activity of 3beta-hydroxysteroid dehydrogenase/isomerase. Endocr Res 24:549-57
Thomas, J L; Evans, B W; Strickler, R C (1997) Affinity radiolabeling identifies peptides associated with the isomerase activity of human type I (placental) 3beta-hydroxysteroid dehydrogenase/isomerase. Biochemistry 36:9029-34
Thomas, J L; Nash, W E; Strickler, R C (1996) Physiological 3 beta-hydroxy-5-ene steroid substrates bind to 3 beta-hydroxysteroid dehydrogenase without the prior binding of cofactor. J Steroid Biochem Mol Biol 58:211-6

Showing the most recent 10 out of 24 publications