Abstract

We have completed the engineering of the BIoMEMS DNA sequencer. Here we propose a reduced engineering activity to support the adoption of the new machine by genomics efforts across the United States. We also propose to customize the new sequencer for DNA applications through the flexibility that comes from a (""""""""chip-based"""""""") MEMS design, and to develop the intrinsic reagent-scaling capability with its potential to significantly affect disposable costs.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Human Genome Research Institute (NHGRI)
Type
Research Project (R01)
Project #
5R01HG001389-09
Application #
6806959
Study Section
Special Emphasis Panel (ZHG1-HGR-N (03))
Program Officer
Schloss, Jeffery
Project Start
1995-09-30
Project End
2006-08-31
Budget Start
2004-09-01
Budget End
2006-08-31
Support Year
9
Fiscal Year
2004
Total Cost
$1,541,799
Indirect Cost

  Institution

Name
Whitehead Institute for Biomedical Research
Department
Type
DUNS #
120989983
City
Cambridge
State
MA
Country
United States
Zip Code
02142

  Publications

McKenna, Brian K; Evans, James G; Cheung, Man Ching et al. (2011) A parallel microfluidic flow cytometer for high-content screening. Nat Methods 8:401-3
Cheung, Man C; Evans, James G; McKenna, Brian et al. (2011) Deep ultraviolet mapping of intracellular protein and nucleic acid in femtograms per pixel. Cytometry A 79:920-32
Ueberfeld, Joern; Ehrlich, Daniel J (2009) Scaling of nucleic acid assays on microelectrophoresis array devices: high-dynamic range multi-gene readout from less than ten transcripts. Electrophoresis 30:2090-9