Current progress from this laboratory has indicated that endotoxin administration impairs myocardial metabolism by two different mechanisms: one by acting through membrane lipid microenvironment and the other by affecting protein phosphorylation/dephosphorylation. The primary aims of this proposal are to continue our search for the understanding of pathophysiology of myocardial dysfunction at the cellular level and, ultimately, to propose a rational remedy for the better management of shock. The specific objectives include studies of phosphorylaton/dephosphorylation of cardiac sarcolemma and sarcoplasmic reticulum in relationship to membrane enzyme and receptor dynamics; investigation of phosphorylation/dephosphorylation of contractile proteins and its association with myofibrillar ATPase activities; studies of the biological activity of calmodulin and the Ca2+ binding properties of calmodulin and triponin C; analysis of the chemical structure and physical properties of myocyte membrane lipids; studies of myocyte-liposome interactions; and development of a liposome preparation to be used as a membrane modifier and as a drug carrier system for the therapeutic interventions of endotoxin shock.

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Project (R01)
Project #
5R01HL030080-05
Application #
3341158
Study Section
Surgery, Anesthesiology and Trauma Study Section (SAT)
Project Start
1982-07-01
Project End
1990-11-30
Budget Start
1988-12-01
Budget End
1989-11-30
Support Year
5
Fiscal Year
1989
Total Cost
Indirect Cost
Name
Saint Louis University
Department
Type
Schools of Medicine
DUNS #
City
Saint Louis
State
MO
Country
United States
Zip Code
63103
Yang, Rei-Cheng; Hsu, Chin; Lee, Tzu-Ying et al. (2013) Transcriptional Regulation of the Group IIA Secretory Phospholipase A2 Gene by C/EBP? in Rat liver and its Relationship to Hepatic Gluconeogenesis during Sepsis. Emerg Med (Los Angel) 3:151
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Dong, L W; Tang, C; Liu, M S (2000) Biphasic redistribution of muscarinic receptor and the altered receptor phosphorylation and gene transcription are underlying mechanisms in the rat heart during sepsis. Cardiovasc Res 45:925-33

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