The shear stress of flowing blood on artery walls and the surfaces of prosthetic devices has a significant influence on the integrity of blood components, the coagulation of blood and formation of thrombi, the production of biochemicals by endothelial cells, the regulation of arterial lumen dimension and the permeability of artery walls to macromolecules and other solutes. In addition, wall shear stress is believed by any to play a role in the pathogenesis of atherosclerosis, an arterial disease which is localized at branch points and bends in arteries. Until recently, knowledge of wall shear stress magnitude and spatial variation in the circulation came primarily from in vitro experiments in rigid models of isolated arterial segments employing Newtonian blood analog fluids. The proposed research investigates the importance of arterial wall elasticity, non-Newtonian blood rheology and the interaction of local and systemic arterial properties in determining the magnitude and distribution of wall shear stress in the cardiovascular system. In the proposed research the following studies will be conducted: 1. Wall shear stress will be measured in dog aortas under normal conditions and altered conditions induced by vasoactive drugs. A hot film anemometer probe designed especially for flush mounting on artery walls will be utilized. 2. Wall shear stress will be measured in elastic models of curved and branched arteries using non-Newtonian blood analog fluids and blood under_physiological flow conditions. Hydraulic impedance elements in a mock circulatory loop will be used to simulate the hemodynamic environment of several arterial segments. 3. Computer simulations of blood flow in arteries which take into account physiologic radial motion of the vessel wall and non- Newtonian blood rheology will be developed. Radial wall motion will be coupled to the pressure pulse through circulatory impedance data, and a power law rheological equation-will model blood. Extensive comparisons between computer simulations and experiments will be made.

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Project (R01)
Project #
5R01HL035549-05
Application #
3349526
Study Section
Surgery and Bioengineering Study Section (SB)
Project Start
1985-12-01
Project End
1991-11-30
Budget Start
1989-12-01
Budget End
1990-11-30
Support Year
5
Fiscal Year
1990
Total Cost
Indirect Cost
Name
Pennsylvania State University
Department
Type
Schools of Engineering
DUNS #
City
University Park
State
PA
Country
United States
Zip Code
16802
Qazi, Henry; Palomino, Rocio; Shi, Zhong-Dong et al. (2013) Cancer cell glycocalyx mediates mechanotransduction and flow-regulated invasion. Integr Biol (Camb) 5:1334-43
Qazi, Henry; Shi, Zhong-Dong; Tarbell, John M (2011) Fluid shear stress regulates the invasive potential of glioma cells via modulation of migratory activity and matrix metalloproteinase expression. PLoS One 6:e20348
Shi, Zhong-Dong; Ji, Xin-Ying; Berardi, Danielle E et al. (2010) Interstitial flow induces MMP-1 expression and vascular SMC migration in collagen I gels via an ERK1/2-dependent and c-Jun-mediated mechanism. Am J Physiol Heart Circ Physiol 298:H127-35
Berardi, Danielle E; Tarbell, John M (2009) Stretch and Shear Interactions Affect Intercellular Junction Protein Expression and Turnover in Endothelial Cells. Cell Mol Bioeng 2:320-331
Shi, Zhong-Dong; Ji, Xin-Ying; Qazi, Henry et al. (2009) Interstitial flow promotes vascular fibroblast, myofibroblast, and smooth muscle cell motility in 3-D collagen I via upregulation of MMP-1. Am J Physiol Heart Circ Physiol 297:H1225-34
Garanich, Jeffrey S; Mathura, Rishi A; Shi, Zhong-Dong et al. (2007) Effects of fluid shear stress on adventitial fibroblast migration: implications for flow-mediated mechanisms of arterialization and intimal hyperplasia. Am J Physiol Heart Circ Physiol 292:H3128-35
Pahakis, Manolis Y; Kosky, Jason R; Dull, Randal O et al. (2007) The role of endothelial glycocalyx components in mechanotransduction of fluid shear stress. Biochem Biophys Res Commun 355:228-33
Dancu, Michael B; Tarbell, John M (2007) Coronary endothelium expresses a pathologic gene pattern compared to aortic endothelium: correlation of asynchronous hemodynamics and pathology in vivo. Atherosclerosis 192:9-14
Dancu, Michael B; Berardi, Danielle E; Vanden Heuvel, John P et al. (2007) Atherogenic Endothelial Cell eNOS and ET-1 Responses to Asynchronous Hemodynamics are Mitigated by Conjugated Linoleic Acid. Ann Biomed Eng 35:1111-9
Dancu, Michael B; Tarbell, John M (2006) Large Negative Stress Phase Angle (SPA) attenuates nitric oxide production in bovine aortic endothelial cells. J Biomech Eng 128:329-34

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