Abstract

Electrophysiological studies will be performed on thalamic neurons using the in vitro thalamic slice preparation and cells acutely dissociated from such slices. The long-term objectives of these experiments are to further define the electrophysiological properties of different classes of thalamic neurons and the details of their responses to acetylcholine and norepinephrine including the involved receptor types and ionic mechanisms for particular responses. Slow synaptic responses evoked by extracellular stimulation in the dorsal lateral geniculate nucleus and other thalamic sites will be examined in terms of involved transmitters, receptors, and ionic conductances. Patch clamp techniques will be used to examine the low threshold Ca++ current in thalamic neurons. Responses to neurotransmitters in lateral geniculate and intralaminar nuclei will be characterized as will alterations in evoked activities following blockage of GABA/A-mediated IPSPs with convulsant agents. The results of these experiments will be relevant to the general issues of the control of cortical excitability and the development of focal or generalized epileptogenesis.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Neurological Disorders and Stroke (NINDS)
Type
Research Project (R01)
Project #
5R01NS006477-23
Application #
3393477
Study Section
Neurology A Study Section (NEUA)
Project Start
1978-01-01
Project End
1994-06-30
Budget Start
1988-07-01
Budget End
1989-06-30
Support Year
23
Fiscal Year
1988
Total Cost
Indirect Cost

  Institution

Name
Stanford University
Department
Type
Schools of Medicine
DUNS #
800771545
City
Stanford
State
CA
Country
United States
Zip Code
94305

  Publications

Gu, Feng; Parada, Isabel; Shen, Fran et al. (2017) Structural alterations in fast-spiking GABAergic interneurons in a model of posttraumatic neocortical epileptogenesis. Neurobiol Dis 108:100-114
Pangratz-Fuehrer, Susanne; Sieghart, Werner; Rudolph, Uwe et al. (2016) Early postnatal switch in GABAA receptor ?-subunits in the reticular thalamic nucleus. J Neurophysiol 115:1183-95
Hiu, Takeshi; Farzampour, Zoya; Paz, Jeanne T et al. (2016) Enhanced phasic GABA inhibition during the repair phase of stroke: a novel therapeutic target. Brain 139:468-80
Takahashi, D Koji; Gu, Feng; Parada, Isabel et al. (2016) Aberrant excitatory rewiring of layer V pyramidal neurons early after neocortical trauma. Neurobiol Dis 91:166-81
Farzampour, Zoya; Reimer, Richard J; Huguenard, John (2015) Endozepines. Adv Pharmacol 72:147-64
Ma, Yunyong; Juntti, Scott A; Hu, Caroline K et al. (2015) Electrical synapses connect a network of gonadotropin releasing hormone neurons in a cichlid fish. Proc Natl Acad Sci U S A 112:3805-10
Farzampour, Zoya; Huguenard, John (2015) Seizing upon mechanisms for impaired consciousness. Neuron 85:453-5
Paz, Jeanne T; Huguenard, John R (2015) Optogenetics and epilepsy: past, present and future. Epilepsy Curr 15:34-8
Prince, David A (2014) How do we make models that are useful in understanding partial epilepsies? Adv Exp Med Biol 813:233-41
Kyuyoung, Christine L; Huguenard, John R (2014) Modulation of short-term plasticity in the corticothalamic circuit by group III metabotropic glutamate receptors. J Neurosci 34:675-87

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