Abstract

The proposed research will determine the patterns of cell proliferation, migration and dispersion of a defined region of the developing murine cerebral cortex, the dorsomedial sector of the E13-E17 cerebral wall. Patterns of cell generation will be determined for the total population of the VZ and SVZ by the method of cumulative labeling with BUdR. Patterns of generation of the """"""""indifferent"""""""" population as well as cells of the astroglial lineage will be discriminated by staining with the cellular antigen marker for radial glial cells, RC2. Parameters of cell production will be based upon the output pattern of a 1 hour cohort of S-phase cells, identified by 3H-thymidine pulse labeling, and followed in 1 hour by a cumulative labeling with BUdR to reveal the total population. The patterns of clonal output and of clonal dispersion will be corroborated by labeling with retroviral transfer of xgal at E12.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Neurological Disorders and Stroke (NINDS)
Type
Research Project (R01)
Project #
5R01NS012005-18
Application #
3394677
Study Section
Neurology B Subcommittee 2 (NEUB)
Project Start
1978-09-26
Project End
1994-03-31
Budget Start
1992-04-01
Budget End
1993-03-31
Support Year
18
Fiscal Year
1992
Total Cost
Indirect Cost

  Institution

Name
Massachusetts General Hospital
Department
Type
DUNS #
City
Boston
State
MA
Country
United States
Zip Code
02199

  Publications

Caviness Jr, Verne S; Nowakowski, Richard S; Bhide, Pradeep G (2009) Neocortical neurogenesis: morphogenetic gradients and beyond. Trends Neurosci 32:443-50
Suter, Bernhard; Nowakowski, Richard S; Bhide, Pradeep G et al. (2007) Navigating neocortical neurogenesis and neuronal specification: a positional information system encoded by neurogenetic gradients. J Neurosci 27:10777-84
Tarui, T; Takahashi, T; Nowakowski, R S et al. (2005) Overexpression of p27 Kip 1, probability of cell cycle exit, and laminar destination of neocortical neurons. Cereb Cortex 15:1343-55
Takahashi, T S; Kinsman, S; Makris, N et al. (2004) Holoprosencephaly--topologic variations in a liveborn series: a general model based upon MRI analysis. J Neurocytol 33:23-35
Caviness Jr, V S; Goto, T; Tarui, T et al. (2003) Cell output, cell cycle duration and neuronal specification: a model of integrated mechanisms of the neocortical proliferative process. Cereb Cortex 13:592-8
Takahashi, T; Kinsman, S; Makris, N et al. (2003) Semilobar holoprosencephaly with midline 'seam': a topologic and morphogenetic model based upon MRI analysis. Cereb Cortex 13:1299-312
Cai, Li; Hayes, Nancy L; Takahashi, Takao et al. (2002) Size distribution of retrovirally marked lineages matches prediction from population measurements of cell cycle behavior. J Neurosci Res 69:731-44
Goto, T; Takahashi, T; Miyama, S et al. (2002) Developmental regulation of the effects of fibroblast growth factor-2 and 1-octanol on neuronogenesis: implications for a hypothesis relating to mitogen-antimitogen opposition. J Neurosci Res 69:714-22
Mitsuhashi, T; Aoki, Y; Eksioglu, Y Z et al. (2001) Overexpression of p27Kip1 lengthens the G1 phase in a mouse model that targets inducible gene expression to central nervous system progenitor cells. Proc Natl Acad Sci U S A 98:6435-40
Miyama, S; Takahashi, T; Goto, T et al. (2001) Continuity with ganglionic eminence modulates interkinetic nuclear migration in the neocortical pseudostratified ventricular epithelium. Exp Neurol 169:486-95

Showing the most recent 10 out of 45 publications