Abstract

This is a revised re-submission of a competitive renewal application to continue studies on the ionic basis of electrical optical activity in dendritic trees of hippocampal pyramidal cells using probes of intracellular ionic activity. Recent experimental work shows that much Ca entry during synaptic activation is through voltage-dependent Ca channels activated subsequent to Na spiking.This proposal will examine whether specific synaptic inputs and agents affecting potassium channels affect the spatial extent of Ca entry. Strong synaptic stimulation can evoke dendritic Ca spikes, and the effects of synaptic inputs and K channel modulators on propagation of dendritic Ca spikes will be studied. An effort will be made to measure Ca entry through NMDA receptor channels. The types of voltage- dependent Ca channels involved in Ca influx will be examined.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Neurological Disorders and Stroke (NINDS)
Type
Research Project (R01)
Project #
2R01NS016295-12A2
Application #
2262973
Study Section
Neurology B Subcommittee 2 (NEUB)
Project Start
1980-04-01
Project End
1998-07-31
Budget Start
1994-08-01
Budget End
1995-07-31
Support Year
12
Fiscal Year
1994
Total Cost
Indirect Cost

  Institution

Name
New York Medical College
Department
Physiology
Type
Schools of Medicine
DUNS #
City
Valhalla
State
NY
Country
United States
Zip Code
10595

  Publications

Ishibashi, Masaru; Gumenchuk, Iryna; Miyazaki, Kenichi et al. (2016) Hypocretin/Orexin Peptides Alter Spike Encoding by Serotonergic Dorsal Raphe Neurons through Two Distinct Mechanisms That Increase the Late Afterhyperpolarization. J Neurosci 36:10097-115
Ross, William N; Miyazaki, Kenichi; Popovic, Marko A et al. (2015) Imaging with organic indicators and high-speed charge-coupled device cameras in neurons: some applications where these classic techniques have advantages. Neurophotonics 2:021005
Miyazaki, Kenichi; Ross, William N (2013) Ca2+ sparks and puffs are generated and interact in rat hippocampal CA1 pyramidal neuron dendrites. J Neurosci 33:17777-88
Ross, William N; Manita, Satoshi (2012) Imaging calcium waves and sparks in central neurons. Cold Spring Harb Protoc 2012:1087-91
Miyazaki, Kenichi; Manita, Satoshi; Ross, William N (2012) Developmental profile of localized spontaneous Ca(2+) release events in the dendrites of rat hippocampal pyramidal neurons. Cell Calcium 52:422-32
Ross, William N (2012) Understanding calcium waves and sparks in central neurons. Nat Rev Neurosci 13:157-68
Manita, Satoshi; Miyazaki, Kenichi; Ross, William N (2011) Synaptically activated Ca2+ waves and NMDA spikes locally suppress voltage-dependent Ca2+ signalling in rat pyramidal cell dendrites. J Physiol 589:4903-20
Fleidervish, Ilya A; Lasser-Ross, Nechama; Gutnick, Michael J et al. (2010) Na+ imaging reveals little difference in action potential-evoked Na+ influx between axon and soma. Nat Neurosci 13:852-60
Manita, Satoshi; Ross, William N (2010) IP(3) mobilization and diffusion determine the timing window of Ca(2+) release by synaptic stimulation and a spike in rat CA1 pyramidal cells. Hippocampus 20:524-39
Zagha, Edward; Manita, Satoshi; Ross, William N et al. (2010) Dendritic Kv3.3 potassium channels in cerebellar purkinje cells regulate generation and spatial dynamics of dendritic Ca2+ spikes. J Neurophysiol 103:3516-25

Showing the most recent 10 out of 44 publications