Abstract

The research is designed to examine the neurotransmitters which are responsible for the postsynaptic inhibition of alpha motoneurons of the lumbar spinal cord during the behavioral state of active sleep. Combined intracellular recording and iontophoretic ejection experiments will be conducted on unanesthetized, undrugged, intact cats during states of sleep and wakefulness. These experiments entail a determination of the neurotransmitters that are involved in various spontaneously-occurring and reticular-induced patterns of postsynaptic inhibition of spinal cord motoneurons during active sleep. The proposed studies should (1) identify the neurotransmitters underlying the different spontaneous patterns of postsynaptic inhibition of lumbarmotoneurons that are present durin the non-rapid eye movement and the rapid eye movement periods of active sleep, (2) identify the neurotransmitters that are involved in th postsynaptic inhibition of lumbar motoneurons that is induced by the nucleus pontis oralis and the nucleus reticularis gigantocellularis during these periods of active sleep, and (3) provide a new experimental paradigm for studying neurotransmitters mediating behavioral process from an intracellular perspective utilizing iontophoretic techniques in the chronic animal.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Neurological Disorders and Stroke (NINDS)
Type
Research Project (R01)
Project #
5R01NS023426-03
Application #
3406885
Study Section
(BPNB)
Project Start
1986-09-01
Project End
1990-08-31
Budget Start
1988-09-01
Budget End
1990-08-31
Support Year
3
Fiscal Year
1988
Total Cost
Indirect Cost

  Institution

Name
University of California Los Angeles
Department
Type
Schools of Medicine
DUNS #
119132785
City
Los Angeles
State
CA
Country
United States
Zip Code
90095

  Publications

Torterolo, P; Sampogna, S; Chase, M H (2011) A restricted parabrachial pontine region is active during non-rapid eye movement sleep. Neuroscience 190:184-93
Pose, I; Sampogna, S; Chase, M H et al. (2011) Nitrergic ventro-medial medullary neurons activated during cholinergically induced active (rapid eye movement) sleep in the cat. Neuroscience 172:246-55
Yamuy, Jack; Fung, Simon J; Xi, Mingchu et al. (2010) State-dependent control of lumbar motoneurons by the hypocretinergic system. Exp Neurol 221:335-45
Engelhardt, John K; Silveira, Valentina; Morales, Francisco R et al. (2010) Serotoninergic control of glycinergic inhibitory postsynaptic currents in rat hypoglossal motoneurons. Brain Res 1345:1-8
Vanini, G; Torterolo, P; McGregor, R et al. (2007) GABAergic processes in the mesencephalic tegmentum modulate the occurrence of active (rapid eye movement) sleep in guinea pigs. Neuroscience 145:1157-67
Morales, F R; Sampogna, S; Rampon, C et al. (2006) Brainstem glycinergic neurons and their activation during active (rapid eye movement) sleep in the cat. Neuroscience 142:37-47
Xi, M-C; Chase, M H (2006) Neuronal mechanisms of active (rapid eye movement) sleep induced by microinjections of hypocretin into the nucleus pontis oralis of the cat. Neuroscience 140:335-42
Torterolo, Pablo; Sampogna, Sharon; Morales, Francisco R et al. (2006) MCH-containing neurons in the hypothalamus of the cat: searching for a role in the control of sleep and wakefulness. Brain Res 1119:101-14
Zhang, Jian-Hua; Sampogna, Sharon; Morales, Francisco R et al. (2005) Age-related ultrastructural changes in hypocretinergic terminals in the brainstem and spinal cord of cats. Neurosci Lett 373:171-4
Zhang, Jian-Hua; Sampogna, Sharon; Morales, Francisco R et al. (2005) Age-related changes of hypocretin in basal forebrain of guinea pig. Peptides 26:2590-6

Showing the most recent 10 out of 65 publications