The long-term objective of the proposed research is to develop an appropriate methodology and test the hypothesis that the heterogeneity of aging is reflected and can be studied at the level of expression profiles of aging tissues. We believe that, on the long term, this approach will help in the systematic deciphering of the multiple basic mechanisms of aging. The main obstacle of a study of the heterogeneity of aging on the level of expression profiling is the high cost, especially because many samples have to be processed. Consequently, the methodological portion of the proposal seeks to develop a low cost, custom array methodology based on membrane immobilized long oligonucleotide probes. We propose to test this methodology on two well-defined biological systems including aging/transgenic heart and cellular replicative senescence. We propose to further use this methodology to accumulate date necessary to test the heterogeneity hypothesis.

Agency
National Institute of Health (NIH)
Institute
National Institute on Aging (NIA)
Type
Small Research Grants (R03)
Project #
1R03AG018536-01
Application #
6197305
Study Section
National Institute on Aging Initial Review Group (NIA)
Program Officer
Kohanski, Ronald A
Project Start
2000-09-01
Project End
2001-08-31
Budget Start
2000-09-01
Budget End
2001-08-31
Support Year
1
Fiscal Year
2000
Total Cost
$87,000
Indirect Cost
Name
Beth Israel Deaconess Medical Center
Department
Type
DUNS #
071723621
City
Boston
State
MA
Country
United States
Zip Code
02215
Coller, Hilary A; Bodyak, Natalya D; Khrapko, Konstantin (2002) Frequent intracellular clonal expansions of somatic mtDNA mutations: significance and mechanisms. Ann N Y Acad Sci 959:434-47
Bodyak, Natalya; Kang, Peter M; Hiromura, Makoto et al. (2002) Gene expression profiling of the aging mouse cardiac myocytes. Nucleic Acids Res 30:3788-94
Coller, H A; Khrapko, K; Bodyak, N D et al. (2001) High frequency of homoplasmic mitochondrial DNA mutations in human tumors can be explained without selection. Nat Genet 28:147-50