Abstract

This proposal is directed at understanding the interaction of Salmonella with host mononuclear phagocytes.
Two Aims are proposed to understand this interaction. The first is to confirm the role of OmpC in bacterial adhesion to macrophages. The gene encoding OmpC will be inactivated by transposon mutagenesis. The ompC gene will be cloned into E. coli, and the OmpC protein will be synthesized and analyzed for biological activity.
The second aim i s to identify macrophage receptors involved in binding S. typhimurium. Separated macrophage proteins will be probed with OmpC. The isolated proteins will be sequenced to obtain information about the receptors on macrophages involved in the recognition of S. typhimurium.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Academic Research Enhancement Awards (AREA) (R15)
Project #
1R15AI037801-01A1
Application #
2074671
Study Section
Bacteriology and Mycology Subcommittee 2 (BM)
Project Start
1996-06-01
Project End
1999-05-31
Budget Start
1996-06-01
Budget End
1999-05-31
Support Year
1
Fiscal Year
1996
Total Cost
Indirect Cost

  Institution

Name
University of New Hampshire
Department
Microbiology/Immun/Virology
Type
Schools of Earth Sciences/Natur
DUNS #
111089470
City
Durham
State
NH
Country
United States
Zip Code
03824

  Publications

Negm, R S; Pistole, T G (1999) The porin OmpC of Salmonella typhimurium mediates adherence to macrophages. Can J Microbiol 45:658-69
Negm, R S; Pistole, T G (1998) Macrophages recognize and adhere to an OmpD-like protein of Salmonella typhimurium. FEMS Immunol Med Microbiol 20:191-9