Abstract

Cholera toxin in vitro catalyses the transfer of ADP-ribose from NAD ion to a number of cellular proteins. These are a) 42K, somtimes 50K, which are regulatory components of adenylate cyclase, b) often 98K, 23K, which share sequences with 42K but whose nature isn't clear, c) even more minor components that have not been studied at all. In the coming year we shall continue to study the minor components, especially 98K, to determine its physiological function and the possible change of function upon ADP-ribosylation. We shall use erythrocytes and enterocytes. We shall also examine non-vertebrate tissues to see what targets, if any, they have, what their sizes are, when they arose, or changed size, and how these changes relate to the evolution of adenylate cyclase and its regulatory systems.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Unknown (R22)
Project #
5R22AI016928-07
Application #
3444524
Study Section
Bacteriology and Mycology Subcommittee 1 (BM)
Project Start
1979-09-01
Project End
1986-08-31
Budget Start
1985-09-01
Budget End
1986-08-31
Support Year
7
Fiscal Year
1985
Total Cost
Indirect Cost

  Institution

Name
Tufts University
Department
Type
Schools of Medicine
DUNS #
604483045
City
Boston
State
MA
Country
United States
Zip Code

  Publications

Popoff, M R; Hauser, D; Boquet, P et al. (1991) Characterization of the C3 gene of Clostridium botulinum types C and D and its expression in Escherichia coli. Infect Immun 59:3673-9
Coburn, J; Kane, A V; Feig, L et al. (1991) Pseudomonas aeruginosa exoenzyme S requires a eukaryotic protein for ADP-ribosyltransferase activity. J Biol Chem 266:6438-46
Gill, D M; Woolkalis, M J (1991) Cholera toxin-catalyzed [32P]ADP-ribosylation of proteins. Methods Enzymol 195:267-80
Coburn, J; Gill, D M (1991) ADP-ribosylation of p21ras and related proteins by Pseudomonas aeruginosa exoenzyme S. Infect Immun 59:4259-62
Hauser, D; Eklund, M W; Kurazono, H et al. (1990) Nucleotide sequence of Clostridium botulinum C1 neurotoxin. Nucleic Acids Res 18:4924
Coburn, J; Dillon, S T; Iglewski, B H et al. (1989) Exoenzyme S of Pseudomonas aeruginosa ADP-ribosylates the intermediate filament protein vimentin. Infect Immun 57:996-8
Coburn, J; Wyatt, R T; Iglewski, B H et al. (1989) Several GTP-binding proteins, including p21c-H-ras, are preferred substrates of Pseudomonas aeruginosa exoenzyme S. J Biol Chem 264:9004-8
Chardin, P; Boquet, P; Madaule, P et al. (1989) The mammalian G protein rhoC is ADP-ribosylated by Clostridium botulinum exoenzyme C3 and affects actin microfilaments in Vero cells. EMBO J 8:1087-92
Tamir, A; Gill, D M (1988) ADP-ribosylation by cholera toxin of membranes derived from brain modifies the interaction of adenylate cyclase with guanine nucleotides and NaF. J Neurochem 50:1791-7
Gill, D M; Coburn, J (1988) ADP-ribosylation of membrane proteins by bacterial toxins in the presence of NAD glycohydrolase. Biochim Biophys Acta 954:65-72

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