Non-Parenchymal Liver Cell Core proposed in this application, will provide to the alcohol research community, highly specialized services of isolation and culture of three types of non-parenchymal cells, sinusoidal endothelial cells, Kupffer cells, and hepatic stellate cells from normal, ethanol-fed, and pair-fed control rats. The principal and co-principal investigators have combined 28 years of experience in isolation of nonparenchymal liver cells and this expertise has served as a unique collaborative tool to support research activities by other investigators on and off campus. For the past two years, this technical resource has been partially subsidized by the NIDDK-funded University of Southern California (USC) Research Center for Liver Disease. However, the Core facility has grown to serve not only 5 USC investigators but also 7 investigators at other universities, and of the total 12 investigators, 10 investigators utilize the Core for alcohol- related research. The main objective of the proposal is to obtain a NIAAA Alcohol Research Resource Award to fully support the activities of the Core with a mission of promoting research on non-parenchymal liver cell biology in alcoholic liver disease (ALD), the field increasingly recognized as a mechanistically important area of research. Isolation of the three types of non-parenchymal liver cells is performed only in several laboratories in the country. Moreover, isolation of the cells from alcohol-fed animals is performed in even fewer laboratories. The Core will not only isolate the cells from normal rats but also work with the Animal Core of the NIAAA-funded USC-UCLA Research Center for Alcoholic Liver and Pancreatic Diseases (Alcohol Research Center), to prepare the cells from the intragastric ethanol infusion model of ALD. It is our goal that these unique services will stimulate the interest of scientists from alcohol and non-alcohol fields in applying novel questions and innovative techniques to research on non-parenchymal liver cell biology in ALD. In addition, the Core will provide scientific advice and hands-on training for the cell isolation procedures, to NIAAA-funded investigators who consider the study of alcohol and non-parenchymal liver cells as their primary interest. These goals will be achieved by close collaborations between the proposed Non- parenchymal Liver Cell Core and te existing Animal and Administrative Core facilities of the Alcohol Research Center, Headquartered at USC and directed by the Principal Investigator.

Agency
National Institute of Health (NIH)
Institute
National Institute on Alcohol Abuse and Alcoholism (NIAAA)
Type
Resource-Related Research Projects (R24)
Project #
1R24AA012885-01
Application #
6224281
Study Section
Health Services Research Review Subcommittee (AA)
Program Officer
Purohit, Vishnu
Project Start
2001-06-01
Project End
2006-05-31
Budget Start
2001-06-01
Budget End
2002-05-31
Support Year
1
Fiscal Year
2001
Total Cost
$144,228
Indirect Cost
Name
University of Southern California
Department
Internal Medicine/Medicine
Type
Schools of Medicine
DUNS #
041544081
City
Los Angeles
State
CA
Country
United States
Zip Code
90089
Khanova, Elena; Wu, Raymond; Wang, Wen et al. (2018) Pyroptosis by caspase11/4-gasdermin-D pathway in alcoholic hepatitis in mice and patients. Hepatology 67:1737-1753
Gajendiran, Priya; Vega, Leonel Iglesias; Itoh, Kie et al. (2018) Elevated mitochondrial activity distinguishes fibrogenic hepatic stellate cells and sensitizes for selective inhibition by mitotropic doxorubicin. J Cell Mol Med 22:2210-2219
Wu, Raymond; Murali, Ramachandran; Kabe, Yasuaki et al. (2018) Baicalein Targets GTPase-Mediated Autophagy to Eliminate Liver Tumor-Initiating Stem Cell-Like Cells Resistant to mTORC1 Inhibition. Hepatology 68:1726-1740
Yang, Zemin; Liu, Yu; Qin, Lan et al. (2017) Cathepsin H-Mediated Degradation of HDAC4 for Matrix Metalloproteinase Expression in Hepatic Stellate Cells: Implications of Epigenetic Suppression of Matrix Metalloproteinases in Fibrosis through Stabilization of Class IIa Histone Deacetylases. Am J Pathol 187:781-797
Machida, Keigo (2017) Existence of cancer stem cells in hepatocellular carcinoma: myth or reality? Hepatol Int 11:143-147
Lai, Keane K Y; Kweon, Soo-Mi; Chi, Feng et al. (2017) Stearoyl-CoA Desaturase Promotes Liver Fibrosis and Tumor Development in Mice via a Wnt Positive-Signaling Loop by Stabilization of Low-Density Lipoprotein-Receptor-Related Proteins 5 and 6. Gastroenterology 152:1477-1491
Zeybel, Müjdat; Luli, Saimir; Sabater, Laura et al. (2017) A Proof-of-Concept for Epigenetic Therapy of Tissue Fibrosis: Inhibition of Liver Fibrosis Progression by 3-Deazaneplanocin A. Mol Ther 25:218-231
Eguchi, Akiko; Lazaro, Raul G; Wang, Jiaohong et al. (2017) Extracellular vesicles released by hepatocytes from gastric infusion model of alcoholic liver disease contain a MicroRNA barcode that can be detected in blood. Hepatology 65:475-490
Chen, Chia-Lin; Huang, Jeffrey Y; Wang, Chun-Hsiang et al. (2017) Hepatitis C virus has a genetically determined lymphotropism through co-receptor B7.2. Nat Commun 8:13882
Li, Yuchang; Lua, Ingrid; French, Samuel W et al. (2016) Role of TGF-? signaling in differentiation of mesothelial cells to vitamin A-poor hepatic stellate cells in liver fibrosis. Am J Physiol Gastrointest Liver Physiol 310:G262-72

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