Abstract

We plan to continue to offer services to both the North American and European scientific communities for MHC typing of macaques. This will include PCR-SSP tests for alleles encoding MHC class I and II molecules that bind peptides derived from SIV. Additionally, we will continue to offer training for individuals who wish to set up MHC typing in their own laboratories. Finally, we will continue to develop a panel of well-characterized cell lines that will be invaluable for the analysis of the MHC in the macaque. To date, we have typed more than 15,000 macaques for approximately 50 investigators using over 114,000 tests. These typings have supported the publication of over 75 manuscripts. The two specific aims of this application are, therefore, to: I. Offer MHC typing of the class I and II loci for investigators working with Indian rhesus macaques. II. Expand our menu for the molecular typing of the macaque's class I and II alleles.

Public Health Relevance

(provided by applicant): SIV infection in macaques is the best animal model to study HIV-infected humans. Furthermore, SIV challenge of vaccinated macaques is critical to HIV vaccine development. Understanding the MHC molecules of the macaque is central to both pathogenesis and vaccine studies.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Resource-Related Research Projects (R24)
Project #
5R24RR016038-10
Application #
7936198
Study Section
National Center for Research Resources Initial Review Group (RIRG)
Program Officer
Contreras, Miguel A
Project Start
2001-02-01
Project End
2014-07-31
Budget Start
2010-08-01
Budget End
2011-07-31
Support Year
10
Fiscal Year
2010
Total Cost
$665,908
Indirect Cost

  Institution

Name
University of Wisconsin Madison
Department
Pathology
Type
Schools of Medicine
DUNS #
161202122
City
Madison
State
WI
Country
United States
Zip Code
53715

  Publications

Jensen, Kara; Dela Pena-Ponce, Myra Grace; Piatak Jr, Michael et al. (2017) Balancing Trained Immunity with Persistent Immune Activation and the Risk of Simian Immunodeficiency Virus Infection in Infant Macaques Vaccinated with Attenuated Mycobacterium tuberculosis or Mycobacterium bovis BCG Vaccine. Clin Vaccine Immunol 24:
Jensen, Kara; Nabi, Rafiq; Van Rompay, Koen K A et al. (2016) Vaccine-Elicited Mucosal and Systemic Antibody Responses Are Associated with Reduced Simian Immunodeficiency Viremia in Infant Rhesus Macaques. J Virol 90:7285-7302
de Groot, Nanine; Doxiadis, Gaby G M; Otting, Nel et al. (2014) Differential recombination dynamics within the MHC of macaque species. Immunogenetics 66:535-44
Van Rompay, Koen K A; Hunter, Zoe; Jayashankar, Kartika et al. (2014) A vaccine against CCR5 protects a subset of macaques upon intravaginal challenge with simian immunodeficiency virus SIVmac251. J Virol 88:2011-24
Reynolds, Matthew R; Weiler, Andrea M; Piaskowski, Shari M et al. (2012) A trivalent recombinant Ad5 gag/pol/nef vaccine fails to protect rhesus macaques from infection or control virus replication after a limiting-dose heterologous SIV challenge. Vaccine 30:4465-75
Otting, Nel; de Groot, Nanine; de Vos-Rouweler, Annemiek J M et al. (2012) Multilocus definition of MHC haplotypes in pedigreed cynomolgus macaques (Macaca fascicularis). Immunogenetics 64:755-65
Vojnov, Lara; Martins, Mauricio A; Bean, Alexander T et al. (2012) The majority of freshly sorted simian immunodeficiency virus (SIV)-specific CD8(+) T cells cannot suppress viral replication in SIV-infected macrophages. J Virol 86:4682-7
Van Rompay, Koen K A; Trott, Kristin A; Jayashankar, Kartika et al. (2012) Prolonged tenofovir treatment of macaques infected with K65R reverse transcriptase mutants of SIV results in the development of antiviral immune responses that control virus replication after drug withdrawal. Retrovirology 9:57
Doxiadis, Gaby G M; de Vos-Rouweler, Annemiek J M; de Groot, Nanine et al. (2012) DR haplotype diversity of the cynomolgus macaque as defined by its transcriptome. Immunogenetics 64:31-7
Mudd, Philip A; Ericsen, Adam J; Burwitz, Benjamin J et al. (2012) Escape from CD8(+) T cell responses in Mamu-B*00801(+) macaques differentiates progressors from elite controllers. J Immunol 188:3364-70

Showing the most recent 10 out of 60 publications