? The rhesus macaque (Macaca mulatta) offers unique opportunities for biomedical research due to similarities to humans in both anatomy and physiology. Vaccine development for HIV and possible bioterrorist agents such as small pox and anthrax require the use of nonhuman primates (NHPs). The rhesus macaque is also the animal of choice for studies of reproductive biology and higher cortical functions. Important studies in these areas are currently impeded by the lack of a critical tool - expression microarrays. The objective of this proposal is to create rhesus macaque expression microarrays by targeted polymerase chain reaction (PCR) amplification of monkey homologs of human genes using information from the human genome project. To maximize scientific and biomedical impact, this targeted approach will be prioritized in three ordered stages. The first stage will be to produce a focused array of 1,800 genes chosen as highest priority by the NHP community. The second stage will be to identify and amplify those monkey genes for which human probes work poorly. The third stage will be to amplify the remaining rhesus macaque genes. Genes already cloned from rhesus cDNA libraries will be excluded from this project. Each stage will have four steps: (1) identify the genes; (2) design primer pairs for targeted amplification of those genes; (3) amplify, subclone, sequence, annotate, and deposit those gene clones; and (4) construct targeted rhesus expression microarrays. This project will produce a resource of major importance to the NHP community. ? ?

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Resource-Related Research Projects (R24)
Project #
5R24RR017444-05
Application #
7237880
Study Section
National Center for Research Resources Initial Review Group (RIRG)
Program Officer
Harding, John D
Project Start
2003-06-18
Project End
2008-04-30
Budget Start
2007-05-01
Budget End
2008-04-30
Support Year
5
Fiscal Year
2007
Total Cost
$445,212
Indirect Cost
Name
University of Nebraska Medical Center
Department
Genetics
Type
Schools of Medicine
DUNS #
168559177
City
Omaha
State
NE
Country
United States
Zip Code
68198
Bimber, Benjamin N; Ramakrishnan, Ranjani; Cervera-Juanes, Rita et al. (2017) Whole genome sequencing predicts novel human disease models in rhesus macaques. Genomics 109:214-220
Cornish, Adam S; Gibbs, Robert M; Norgren Jr, Robert B (2016) Exome screening to identify loss-of-function mutations in the rhesus macaque for development of preclinical models of human disease. BMC Genomics 17:170
Sandler, Netanya G; Bosinger, Steven E; Estes, Jacob D et al. (2014) Type I interferon responses in rhesus macaques prevent SIV infection and slow disease progression. Nature 511:601-5
Norgren Jr, Robert B (2013) Improving genome assemblies and annotations for nonhuman primates. ILAR J 54:144-53
Zhang, Xiongfei; Goodsell, Joel; Norgren Jr, Robert B (2012) Limitations of the rhesus macaque draft genome assembly and annotation. BMC Genomics 13:206
Duan, Fenghai; Spindel, Eliot R; Li, Yu-Hua et al. (2007) Intercenter reliability and validity of the rhesus macaque GeneChip. BMC Genomics 8:61
Ferguson, Betsy; Street, Summer L; Wright, Hollis et al. (2007) Single nucleotide polymorphisms (SNPs) distinguish Indian-origin and Chinese-origin rhesus macaques (Macaca mulatta). BMC Genomics 8:43
Spindel, Eliot R; Pauley, Mark A; Jia, Yibing et al. (2005) Leveraging human genomic information to identify nonhuman primate sequences for expression array development. BMC Genomics 6:160