Abstract

This laboratory is involved in the isolation and characterization of oncogenes. In particular, we are conducting a molecular analysis of action of the ras proteins. Both the immediate and long-range targets of action of these proteins, the """"""""ras pathway,"""""""" are being determined and the interconnection of this pathway with known pathways is being studied. The yeast S. cerevisiae has ras genes which are structurally, biochemically, and functionally closely related to the mammalian ras genes. Preliminary work strongly suggests that the yeast ras genes exert their effects by modulating cyclic AMP levels. Studies of the yeast ras will help guide studies of the mammalian ras. In addition, we propose to utilize recent advances in techniques of gene transfer to search for genes which function to restrain cellular proliferation. (Y)

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Unknown (R35)
Project #
5R35CA039829-06
Application #
3479151
Study Section
Special Emphasis Panel (SRC)
Project Start
1985-07-01
Project End
1992-06-30
Budget Start
1990-07-01
Budget End
1991-06-30
Support Year
6
Fiscal Year
1990
Total Cost
Indirect Cost

  Institution

Name
Cold Spring Harbor Laboratory
Department
Type
DUNS #
065968786
City
Cold Spring Harbor
State
NY
Country
United States
Zip Code
11724

  Publications

Mu, David; Chen, Liyun; Zhang, Xiping et al. (2003) Genomic amplification and oncogenic properties of the KCNK9 potassium channel gene. Cancer Cell 3:297-302
Hamaguchi, Masaaki; Meth, Jennifer L; von Klitzing, Christine et al. (2002) DBC2, a candidate for a tumor suppressor gene involved in breast cancer. Proc Natl Acad Sci U S A 99:13647-52
Wen, S; Stolarov, J; Myers, M P et al. (2001) PTEN controls tumor-induced angiogenesis. Proc Natl Acad Sci U S A 98:4622-7
Lucito, R; West, J; Reiner, A et al. (2000) Detecting gene copy number fluctuations in tumor cells by microarray analysis of genomic representations. Genome Res 10:1726-36
Dong, D L; Liu, R; Sherlock, R et al. (1999) Molecular forceps from combinatorial libraries prevent the farnesylation of Ras by binding to its carboxyl terminus. Chem Biol 6:133-41
Hamaguchi, M; O'Connor, E A; Chen, T et al. (1998) Rapid isolation of cDNA by hybridization. Proc Natl Acad Sci U S A 95:3764-9
Lucito, R; Nakimura, M; West, J A et al. (1998) Genetic analysis using genomic representations. Proc Natl Acad Sci U S A 95:4487-92
Li, J; Yen, C; Liaw, D et al. (1997) PTEN, a putative protein tyrosine phosphatase gene mutated in human brain, breast, and prostate cancer. Science 275:1943-7
Xu, H P; Yanak, B L; Wigler, M H et al. (1996) New polymorphic markers in the vicinity of the pearl locus on mouse chromosome 13. Mamm Genome 7:16-9
Field, J; Xu, H P; Michaeli, T et al. (1990) Mutations of the adenylyl cyclase gene that block RAS function in Saccharomyces cerevisiae. Science 247:464-7

Showing the most recent 10 out of 11 publications