Transposable elements (TEs) are omnipresent genomic parasites, comprising >70% of the nuclear DNA in certain lineages. In addition to producing deleterious mutations, TEs can exert lethal, genotoxic effects on host cells by causing double-stranded breaks during insertion and excision. Host genomes have two mechanisms for avoiding the fitness costs of parasitic TEs: resistance and tolerance. Mechanisms of resistance reduce TE load by repressing transposition, while mechanisms of tolerance reduce the fitness consequences of existing TEs. While resistance to TEs is studied extensively, with small-RNAs in particular emerging as a taxonomically widespread strategy, tolerance is a new concept that I have recently proposed. Furthermore, through an innovative QTL mapping experiment, I provided critical proof-of-principle for tolerant genetic variants in Drosophila melanogaster. In the future, I propose to build on these recent discoveries to expand our understanding of tolerance mechanisms and evolution. First, we will study the mechanism and recent evolutionary history of bruno-dependent tolerance to P- element DNA transposons. Bruno is a developmental regulator of oogenesis, which we have recently demonstrated is a major determinant of female germline tolerance to unregulated P-element transposition. By combining genetic analysis of mutant alleles, as well tolerant bruno variants we have isolated from natural populations, we will reveal the underlying mechanism of bruno-dependent tolerance. Furthermore, by taking advantage of the unique opportunity provided by historic D. melanogaster collections, we will evaluate the contribution of bruno tolerant variants to the host adaptation to the P-element invasion in the mid 20th century. My second research direction is motivated by our discovery that natural variation in heterochromatin is an important determinant of how cells are impacted by unregulated transposition: larger doses of heterochromatin and reduced heterochromatin formation decrease cellular tolerance. These observations are reminiscent of Barbara McClintock's ?genomic shock? model, in which tremors arising from DNA damage or cellular stress trigger the release normally quiescent repeats. I therefore propose to interrogate the relationship between heterochromatic variation and response to genotoxic stress at both the cellular and regulatory levels. My long-term goal is to reveal complex and intimate relationships between TEs and their hosts, which contribute to genome evolution and shape the evolution of gametogenesis. A better understanding of these interactions is of vital importance because: 1) they are the interface at which TEs and host cells coevolve, 2) TE activity is implicated in the onset, progression of many tumor types and age related neurodegenerative diseases, and 3) vast differences in TE content and distribution between host genomes remain largely unexplained.
Transposable elements are mobile DNA sequences which spread through eukaryotic genomes, making up ~50% of the human genome. The mobilization of transposable elements is implicated in the onset and progression of many cancers, as well as age-related neurodegenerative diseases. The proposed research uses Drosophila melanogaster as a model to reveal the mechanisms and evolution of host cellular responses to transposon mobilization.
