Our long term goal is to elucidate the mechanisms of initiation and regulation of RNA polymerase II transcription. Our experimental approach is to reconstitute these processes with purified proteins from yeast, and bring the power of combined biochemical and genetic analysis to bear on the mechanisms. During the previous project period, we developed a fully defined, appropriately regulated transcription system. The sub cloning of genes for all components and expression of many of the proteins in recombinant form should enable the culmination of our efforts during the coming project period.
Specific aims of the proposed research are as follows: 1. Reconstitution and functional analysis of TFIIH. 2. Reconstitution and functional analysis of mediator. 3. Characterization of mammalian mediator. 4. Reconstitution of yeast TAF-dependent transcription in vitro. 5. Studies of RSC-nucleosome interaction.
| Lorch, Yahli; Maier-Davis, Barbara; Kornberg, Roger D (2018) Histone Acetylation Inhibits RSC and Stabilizes the +1 Nucleosome. Mol Cell 72:594-600.e2 |
| Nagai, Shigeki; Davis, Ralph E; Mattei, Pierre Jean et al. (2017) Chromatin potentiates transcription. Proc Natl Acad Sci U S A 114:1536-1541 |
| Eagen, Kyle P; Aiden, Erez Lieberman; Kornberg, Roger D (2017) Polycomb-mediated chromatin loops revealed by a subkilobase-resolution chromatin interaction map. Proc Natl Acad Sci U S A 114:8764-8769 |
| Robinson, Philip J; Trnka, Michael J; Bushnell, David A et al. (2016) Structure of a Complete Mediator-RNA Polymerase II Pre-Initiation Complex. Cell 166:1411-1422.e16 |
| Lorch, Yahli; Kornberg, Roger D (2015) Chromatin-remodeling and the initiation of transcription. Q Rev Biophys 48:465-70 |
| Guan, Shenheng; Trnka, Michael J; Bushnell, David A et al. (2015) Deconvolution method for specific and nonspecific binding of ligand to multiprotein complex by native mass spectrometry. Anal Chem 87:8541-6 |
| Eagen, Kyle P; Hartl, Tom A; Kornberg, Roger D (2015) Stable Chromosome Condensation Revealed by Chromosome Conformation Capture. Cell 163:934-46 |
| Fazal, Furqan M; Meng, Cong A; Murakami, Kenji et al. (2015) Real-time observation of the initiation of RNA polymerase II transcription. Nature 525:274-7 |
| Lu, Jonathan; Trnka, Michael J; Roh, Soung-Hun et al. (2015) Improved Peak Detection and Deconvolution of Native Electrospray Mass Spectra from Large Protein Complexes. J Am Soc Mass Spectrom 26:2141-51 |
| Murakami, Kenji; Mattei, Pierre-Jean; Davis, Ralph E et al. (2015) Uncoupling Promoter Opening from Start-Site Scanning. Mol Cell 59:133-8 |
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