The goal of this proposal is to create a simple, accurate and low cost method for drug susceptibility profiling HIV-positive patients. It is proposed to use linear signal amplification to rapidly detect unique nucleic acid sequences that may be present as a small fraction (<0.1 - 10%) of the virus population. The PI contends that this makes the method well suited for detection of targeted mutations in the variable genetic background characteristic of HIV. This phase I study describes detection of 4 highly prevalent HIV mutations in the pol gene that reduce susceptibility to widely used reverse transcriptase inhibitors. The proposal includes plans to validate the methodology on clinical samples.

Proposed Commercial Applications

NOT AVAILABLE

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Small Business Innovation Research Grants (SBIR) - Phase I (R43)
Project #
1R43AI043117-01
Application #
2646422
Study Section
Special Emphasis Panel (ZRG5-ARRC (01))
Project Start
1998-05-01
Project End
1998-10-30
Budget Start
1998-05-01
Budget End
1998-10-30
Support Year
1
Fiscal Year
1998
Total Cost
Indirect Cost
Name
Third Wave Technologies, Inc.
Department
Type
DUNS #
City
Madison
State
WI
Country
United States
Zip Code
53719