Polymerase chain reaction (PCR) amplification and detection in situ has been described for the human papillomavirus (HPV 16, 1) and visna virus (2). Use of PCR techniques is extremely powerful yet has some disadvantages. PCR amplification in situ circumvents some of these and also allows correlation of cell morphology with mRNA expression or presence of a translocation. In Phase I, methods of PCR amplification in situ for cancer research, using formalin fixed, paraffin embedded cell lines will be optimized. These targets will be the t(14;18) bcl2 (MBR)/IgH translocation using SU-DHL-6 cells, and RNA-PCR in situ for the t(9;22) bcr/abl translocation, using K562 cells. The HL-60 cell line will be used as a negative control for mixing experiments. In Phase II and III, assays for the t(14;18) bcl2 (MCR)/IgH t(9;22) bcr/abl (ALL), t(1;19) E2A/prl-pbx, t(15;17)myl/RARa, and t(6;9) dek/can translocation will be developed and commercialized.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Small Business Innovation Research Grants (SBIR) - Phase I (R43)
Project #
1R43CA057044-01
Application #
3493086
Study Section
Special Emphasis Panel (SSS (B1))
Project Start
1992-09-30
Project End
1993-03-30
Budget Start
1992-09-30
Budget End
1993-03-30
Support Year
1
Fiscal Year
1992
Total Cost
Indirect Cost
Name
Osi Pharmaceuticals, Inc.
Department
Type
DUNS #
City
Uniondale
State
NY
Country
United States
Zip Code
11553