The larger objective of the present proposal is to commercialize a small molecule screening technology that can vastly improve the rate at which new lead candidates are brought to market. Here we propose to refine a technology allowing massively parallel multiplex (MPM) screens for small molecules that address multiple targets simultaneously, to a commercializable screening format. The approach has the power to increase the output from a single screening operation by orders of magnitude. It has the additional benefit of defining not only hits but, simultaneously, the specificity of the hits to a set of biological pathways. Phase I of this project will implement three defined improvements to the technology (Goal 1) and validate the technology using a clinically relevant chemotherapeutic and human glioma cells (Goal 2).Narrative The technology to be exploited here seeks to tap the commercial potential of an extremely broad based biological screen that will identify chemical compounds that act as bio-probes or lead compounds for drug development across a broad range of molecular targets in the cell. In this phase of the work, identification of genes known to be involved in mediating the clinically observed biological responses to a chemotherapeutic agent will demonstrate that the application of this approach to a high throughput format is likely to yield disease relevant reagents. ? ? ?

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Small Business Innovation Research Grants (SBIR) - Phase I (R43)
Project #
1R43CA112827-01A2
Application #
7406527
Study Section
Special Emphasis Panel (ZRG1-ONC-V (13))
Program Officer
Haim, Todd E
Project Start
2008-04-01
Project End
2009-09-30
Budget Start
2008-04-01
Budget End
2009-09-30
Support Year
1
Fiscal Year
2008
Total Cost
$132,000
Indirect Cost
Name
Buffalo Molecular Target Laboratory
Department
Type
DUNS #
610557923
City
Buffalo
State
NY
Country
United States
Zip Code
14263