Currently, there exist a number of methods for the study of transcription factors and gene-promoter activity. These include reporter gene assays, gel shift assays, and expression-profiling methods such as microarray analysis and SAGE. These methods are well established and have been used to generate a wealth of information. Accordingly, there are many commercially available kits designed to facilitate and speed these analyses. However, none of the above methods enables identification of the DNA targets that are actually bound by a particular transcription factor in a particular population of cells. The current best method to generate this type of transcription """"""""snapshot"""""""" is the Chromatin Immunoprecipitation (CHIP) assay. This method is technically demanding and is not well supported by commercially available complete kits. Similarly, a powerful extension of the CHIP assay, specifically, the merging of the CHIP assay with microarray analysis of the immunoprecipitated DNA, is not available commercially. We propose to develop reagents to simplify and extend use of the CHIP assay for the study of transcription factor/DNA interactions in living mammalian cells. The long-term objective of this proposal is the development and commercialization of novel kits and reagents that speed the study of in vivo interactions between transcription factors and DNA.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Small Business Innovation Research Grants (SBIR) - Phase I (R43)
Project #
1R43GM068174-01
Application #
6643757
Study Section
Special Emphasis Panel (ZRG1-SSS-2 (10))
Program Officer
Ikeda, Richard A
Project Start
2003-04-01
Project End
2003-09-30
Budget Start
2003-04-01
Budget End
2003-09-30
Support Year
1
Fiscal Year
2003
Total Cost
$99,850
Indirect Cost
Name
Active Motif, Inc.
Department
Type
DUNS #
109145701
City
Carlsbad
State
CA
Country
United States
Zip Code
92008