Recent developments in near-field instrumentation have provided PRAIRIE TECHNOLOGIES with the necessary understanding to develop a multi-mode confocal microscope that will integrate confocal microscopy with simultaneous high-speed fluorescence measurements as well as sub-diffraction resolution imaging. This powerful new tool will revolutionize scientists' ability to study neuronal function and dysfunction in living systems. In phase I we will build and test an alpha prototype by integrating a high-speed fluorescence detection path into our existing confocal microscope. In phase II, we will use the knowledge gained in this phase I feasibility to design and build a commercial simultaneous near-field and confocal imaging system. The resultant product will permit the scientist to address significant questions concerning ion fluxes, synaptic transmission and the molecular nature of learning and memory.
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