The University of Texas Protein Sequencing Center operates a Beckman model 890C spinning cup sequencer. This instrument is incapable of satisfactorily determining sequences in the 10-500 picomole range. The gas-phase sequencer (Applied Biosystems) operates successfully in this range. The Sequencing Center requires this instrument in order to be able to serve the needs of the strong group in molecular biology and biochemistry on the Campus. It is essential that we be able to provide NH2-terminal sequences of proteins from single individual bands in an acrylamide gel2. We cannot do this now. The Center has become well-established over the last five years as a provider of both amino acid analyses and of amino acid sequences to the research community. Acquisition of a Gas-phase Sequencer would enable the Center to perform its function with state-of-the-art equipment of the necessary sensitivity.

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Unknown (S03)
Project #
2S03RR003335-13
Application #
2282370
Study Section
Special Emphasis Panel (NSS)
Project Start
1983-03-01
Project End
1996-02-29
Budget Start
1995-04-01
Budget End
1996-02-29
Support Year
13
Fiscal Year
1995
Total Cost
Indirect Cost
Name
University of Maryland Balt CO Campus
Department
Type
Schools of Arts and Sciences
DUNS #
City
Baltimore
State
MD
Country
United States
Zip Code
21250