Glutamic oxalacetic transaminase (GOT, aspartate aminotransferase EC 2.6.1.1) catalyzes reversibly the transamination of the amino, -NH2, group of L-aspartic acid to a- letoglutaric acid with the subsequent formation of oxalacetic acid and L-glutamic acid, respectively. Detection of the enzyme activity in blood serum is used for clinical diagnosis of myocardial infraction, hepatitis and other diseases. Therefore, the availability of sensitive, accurate and reproducible methods for the assay of GOT activity is of prime clinical importance. The research proposes the development of new analytical method in which both spectrophotometeic and potentiometric techniques will be applied for the assay of GOT activity in flow-injection system for clinical purposes. L-aspartate produced during the more favorable GOT catalyzed reaction can be converted to ammonium ion and funarate in the presence of L-aspartase. The ammonium ion, subsequently, can be detected spectrophotometrically and potentiometrically using ammonium ion selective electrodes. The concentration of ammonium ion is directly related to GOT activity. The application of two electrodes of the same kind; as reference and indicating electrodes; in flow system increases the sensitivity and minimizes interferences. The suggested methods are specific, accurate and sensitive. The proposed research also provide means and valuable information for kinetic studies of the GOT enzyme system.

Project Start
Project End
Budget Start
Budget End
Support Year
20
Fiscal Year
1990
Total Cost
Indirect Cost
Name
Jackson State University
Department
Type
DUNS #
044507085
City
Jackson
State
MS
Country
United States
Zip Code
39217
Beqa, Lule; Fan, Zhen; Singh, Anant Kumar et al. (2011) Gold nano-popcorn attached SWCNT hybrid nanomaterial for targeted diagnosis and photothermal therapy of human breast cancer cells. ACS Appl Mater Interfaces 3:3316-24
Khan, Sadia Afrin; Singh, Anant K; Senapati, Dulal et al. (2011) Targeted highly sensitive detection of multi-drug resistant Salmonella DT104 using gold nanoparticles. Chem Commun (Camb) 47:9444-6
Singh, Anant K; Lu, Wentong; Senapati, Dulal et al. (2011) Long-range nanoparticle surface-energy-transfer ruler for monitoring photothermal therapy response. Small 7:2517-25
Huang, Yong; Zhao, Shulin; Shi, Ming et al. (2010) Chemiluminescent immunoassay of thyroxine enhanced by microchip electrophoresis. Anal Biochem 399:72-7
Wang, Shuguang; Singh, Anant K; Senapati, Dulal et al. (2010) Rapid colorimetric identification and targeted photothermal lysis of Salmonella bacteria by using bioconjugated oval-shaped gold nanoparticles. Chemistry 16:5600-6
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Lu, Wentong; Arumugam, Sri Ranjini; Senapati, Dulal et al. (2010) Multifunctional oval-shaped gold-nanoparticle-based selective detection of breast cancer cells using simple colorimetric and highly sensitive two-photon scattering assay. ACS Nano 4:1739-49
Dadiboyena, Sureshbabu; Valente, Edward J; Hamme 2nd, Ashton T (2010) Synthesis of Novel Pyrazoles via [2+3]-Dipolar Cycloaddition Using Alkyne Surrogates. Tetrahedron Lett 51:1341
Stewart, Gernerique; Smith, Keonia; Chornes, Ashley et al. (2010) PHOTOCHEMICAL REACTION OF NITRO-POLYCYCLIC AROMATIC HYDROCARBONS: EFFECT BY SOLVENT AND STRUCTURE. Environ Chem Lett 8:301-306
Zhao, Shulin; Huang, Yong; Shi, Ming et al. (2010) Chemiluminescence resonance energy transfer-based detection for microchip electrophoresis. Anal Chem 82:2036-41

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