The investigator proposes an experimental-theoretical program to investigate the mechanism of a charge transfer in proteins and protein lipid (membrane) systems, DNA, RNA, etc. Small molecule-protein interaction form the overture to many elementary physiological processes at the cellular level. The primary manifestation of these interactions is very frequently either an immediate alteration in the membrane potential or a significant rearrangement in the intraprotein charge distribution. In both cases the transport properties of electrical charge carriers in the protein are supposed to change. Solid state physical measurements on proteins are believed to furnish valuable information regarding charge carrier injection into, and movement in, proteins. Thin protein films may offer a unique opportunity to extend this experimental approach towards proteins which are available only in very small quantities. The most recent work shows that: 1) Only a finite number of sites may exist on the surface of protein molecules through which charges carriers can be preferentially injected (ejected) from (into) the electrode into (from) the protein. Similar considerations may be true for the interprotein charge transfer as well. 2) The number of these charge carrier transfer sites must be relatively small, the (partial) blocking of one or two of them per protein being detectable. 3) The preferential charge carrier transfer sites may not be completely equivalent to each other due to either possible minor differences in their actual composition and structure, or the differences in their positions with respects to the injecting electrode and/or to neighboring proteins and their corresponding charge carrier transfer sites. 4) Primary amine groups are probably essential components in the formation of the preferential charge carrier transfer sites on proteins.

Project Start
Project End
Budget Start
Budget End
Support Year
20
Fiscal Year
1990
Total Cost
Indirect Cost
Name
Jackson State University
Department
Type
DUNS #
044507085
City
Jackson
State
MS
Country
United States
Zip Code
39217
Beqa, Lule; Fan, Zhen; Singh, Anant Kumar et al. (2011) Gold nano-popcorn attached SWCNT hybrid nanomaterial for targeted diagnosis and photothermal therapy of human breast cancer cells. ACS Appl Mater Interfaces 3:3316-24
Khan, Sadia Afrin; Singh, Anant K; Senapati, Dulal et al. (2011) Targeted highly sensitive detection of multi-drug resistant Salmonella DT104 using gold nanoparticles. Chem Commun (Camb) 47:9444-6
Singh, Anant K; Lu, Wentong; Senapati, Dulal et al. (2011) Long-range nanoparticle surface-energy-transfer ruler for monitoring photothermal therapy response. Small 7:2517-25
Huang, Yong; Zhao, Shulin; Shi, Ming et al. (2010) Chemiluminescent immunoassay of thyroxine enhanced by microchip electrophoresis. Anal Biochem 399:72-7
Wang, Shuguang; Singh, Anant K; Senapati, Dulal et al. (2010) Rapid colorimetric identification and targeted photothermal lysis of Salmonella bacteria by using bioconjugated oval-shaped gold nanoparticles. Chemistry 16:5600-6
Lu, Wentong; Singh, Anant Kumar; Khan, Sadia Afrin et al. (2010) Gold nano-popcorn-based targeted diagnosis, nanotherapy treatment, and in situ monitoring of photothermal therapy response of prostate cancer cells using surface-enhanced Raman spectroscopy. J Am Chem Soc 132:18103-14
Lu, Wentong; Arumugam, Sri Ranjini; Senapati, Dulal et al. (2010) Multifunctional oval-shaped gold-nanoparticle-based selective detection of breast cancer cells using simple colorimetric and highly sensitive two-photon scattering assay. ACS Nano 4:1739-49
Dadiboyena, Sureshbabu; Valente, Edward J; Hamme 2nd, Ashton T (2010) Synthesis of Novel Pyrazoles via [2+3]-Dipolar Cycloaddition Using Alkyne Surrogates. Tetrahedron Lett 51:1341
Stewart, Gernerique; Smith, Keonia; Chornes, Ashley et al. (2010) PHOTOCHEMICAL REACTION OF NITRO-POLYCYCLIC AROMATIC HYDROCARBONS: EFFECT BY SOLVENT AND STRUCTURE. Environ Chem Lett 8:301-306
Zhao, Shulin; Huang, Yong; Shi, Ming et al. (2010) Chemiluminescence resonance energy transfer-based detection for microchip electrophoresis. Anal Chem 82:2036-41

Showing the most recent 10 out of 119 publications