Abstract

The long range objectives for this project are to better understand the mechanism of ligand binding in heme proteins using ruthenium porphyrins and ruthenium substituted myoglobin and hemoglobin as model systems for the naturally occurring proteins. A broad understanding of oxygen binding in hemoglobin will provide information to better deal with genetic diseases involving hemoglobin.
The specific aims of the project are two fold. First, the ligand exchange kinetics, electrochemistry, and spectroscopy of a series of ruthenium(II) porphyrins will be studied in order to determine the mode of bonding of ligands to ruthenium porphyrins. This is important to an understanding of how these ruthenium porphyrins function when incorporated into hemoglobin. Second, a series of ruthenium substituted heme proteins will be prepared and their CO and O2 affinities studied in order to learn more about the mechanism of ligand binding in native hemoglobin.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Minority Biomedical Research Support - MBRS (S06)
Project #
5S06GM008101-23
Application #
3755842
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
23
Fiscal Year
1994
Total Cost
Indirect Cost

  Institution

Name
California State University Los Angeles
Department
Type
DUNS #
City
Los Angeles
State
CA
Country
United States
Zip Code
90032

  Publications

Durvasula, Ramani S (2017) Personality Disorders and Health: Lessons Learned and Future Directions. Behav Med 43:227-232
Crespo, Noe C; Yoo, Eun Jung; Hawkins, Steven A (2011) Anthropometric and lifestyle associations of bone mass in healthy pre-menopausal Mexican and Asian American women. J Immigr Minor Health 13:74-80
Kumar, Satish; Velasco, Kriya; McCurdy, Alison (2010) X-ray, kinetics and DFT studies of photochromic substituted benzothiazolinic spiropyrans. J Mol Struct 968:13-18
Jiang, Dianlu; Li, Xiangjun; Williams, Renee et al. (2009) Ternary complexes of iron, amyloid-beta, and nitrilotriacetic acid: binding affinities, redox properties, and relevance to iron-induced oxidative stress in Alzheimer's disease. Biochemistry 48:7939-47
Pech, Herbe; Henry, Amanda; Khachikian, Crist S et al. (2009) Detection of geothermal phosphite using high-performance liquid chromatography. Environ Sci Technol 43:7671-5
Jiang, Dianlu; Dinh, Kim Lien; Ruthenburg, Travis C et al. (2009) A kinetic model for beta-amyloid adsorption at the air/solution interface and its implication to the beta-amyloid aggregation process. J Phys Chem B 113:3160-8
Hong, Hyun-Seok; Rana, Sandeep; Barrigan, Lydia et al. (2009) Inhibition of Alzheimer's amyloid toxicity with a tricyclic pyrone molecule in vitro and in vivo. J Neurochem 108:1097-1108
Kumar, Satish; Chau, Cindy; Chau, Gordan et al. (2008) Synthesis and metal complexation properties of bisbenzospiropyran chelators in water. Tetrahedron 64:7097-7105
Kumar, Satish; Hernandez, David; Hoa, Brenda et al. (2008) Synthesis, photochromic properties, and light-controlled metal complexation of a naphthopyran derivative. Org Lett 10:3761-4
Valentine, Sonya C; Contreras, Deisy; Tan, Stephanie et al. (2008) Phenotypic and molecular characterization of Acinetobacter baumannii clinical isolates from nosocomial outbreaks in Los Angeles County, California. J Clin Microbiol 46:2499-507

Showing the most recent 10 out of 42 publications