We recently described spontaneous cataract formation in the Dahl salt-sensitive rat. Preliminary data demonstrating a decreased incidence of cataract development when salt-sensitive rats are maintained on a normal, rather than high sodium diet suggests that cataractogenesis may be linked to hypertension in this genetic model. In addition, our finding of altered lenticular and aqueous humor electrolyte composition in salt-sensitive rats with cataracts, suggests the possibility that cataract formation may be the consequence of altered lenticular ionic transport. We now propose to evaluate this possibility through measurements of lenticular sodium and potassium transport in salt-sensitive rats with cataractous lesions. Passive ionic fluxes will be assessed through studies of lenticular 22Na uptake and 86Rb efflux. Active sodium and potassium transport will also be determined through 22Na efflux and 86Rb uptake studies. Furthermore, we propose to evaluate the possibility that changes in ionic transport may precede cataract formation, and that such changes may also parallel the development of hypertension in the salt-sensitive rat. To this end' longitudinal studies of lenticular ionic transport will be carried out in rats beginning at weanling age. Although a defect in lenticular calcium transport could also explain our observations, normal lenticular calcium transport processes have not been clearly defined, and calcium flux studies in the lens are of limited value. Nevertheless, we will examine the possibility that cataract formation may be associated with increased lenticular calcium permeability through measurements of 45Ca uptake. Finally, we propose to determine if cataracts can be prevented by preventing the development of hypertension in salt-sensitive rats. In this manner we will determine if elevated blood pressure, per se, is a necessary condition for cataract formation. Our observations of cataracts associated with hypertension is novel to both ophthalmic and hypertension research. The proposed studies address major issues that are central to understanding the possible relationship between cataractogenesis and hypertensive disease.
| Ramírez-Camejo, Luis A; Maldonado-Morales, Génesis; Bayman, Paul (2017) Differential Microbial Diversity in Drosophila melanogaster: Are Fruit Flies Potential Vectors of Opportunistic Pathogens? Int J Microbiol 2017:8526385 |
| Morales-Rivera, Keyla F; Piñero Cruz, Dalice M; Prieto, Jose A (2017) Crystal structure of (-)-(S)-4-[(2S,3S,4S,Z)-3-hydroxy-4-methyl-hept-5-en-2-yl]-1,3-dioxolan-2-one. Acta Crystallogr E Crystallogr Commun 73:1070-1072 |
| García-Arriaga, Marilyn; Acosta-Santiago, Maxier; Cruz, Antony et al. (2017) Probing the Limits of Supramolecular G-Quadruplexes Using Atomistic Molecular Dynamics Simulations. Inorganica Chim Acta 468:209-222 |
| Huang, Qing; Quiñones, Edwin (2016) A spectroscopic method to determine the activity of the restriction endonuclease EcoRV that involves a single reaction. Anal Biochem 497:103-5 |
| Mège, Pascal; Schizas, Nikolaos V; Reyes, Joselyd García et al. (2015) Genetic seascape of the threatened Caribbean elkhorn coral, Acropora palmata, on the Puerto Rico Shelf. Mar Ecol (Berl) 36:195-209 |
| Morales-Rivera, Amarilys; Hernández-Burgos, Mayté M; Martínez-Rivera, Arlene et al. (2014) Anxiolytic effects of oxytocin in cue-induced cocaine seeking behavior in rats. Psychopharmacology (Berl) 231:4145-55 |
| Ramírez-Camejo, Luis A; Torres-Ocampo, Ana P; Agosto-Rivera, José L et al. (2014) An opportunistic human pathogen on the fly: strains of Aspergillus flavus vary in virulence in Drosophila melanogaster. Med Mycol 52:211-9 |
| Martínez-Rivera, Arlene; Rodríguez-Borrero, Enrique; Matías-Alemán, María et al. (2013) Metabotropic glutamate receptor 5 within nucleus accumbens shell modulates environment-elicited cocaine conditioning expression. Pharmacol Biochem Behav 110:154-60 |
| Galindo-Cardona, Alberto; Acevedo-Gonzalez, Jenny P; Rivera-Marchand, Bert et al. (2013) Genetic structure of the gentle Africanized honey bee population (gAHB) in Puerto Rico. BMC Genet 14:65 |
| Santiago-Rodriguez, Tasha M; Rivera, Jessica I; Coradin, Mariel et al. (2013) Antibiotic-resistance and virulence genes in Enterococcus isolated from tropical recreational waters. J Water Health 11:387-96 |
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