Fungal infections are killing sea fans (Gorgonia spp.) all over the Caribbean, with grave consequences for health of coral reefs. These infections are aspergillosis--that is, the pathogen is Aspergillus--and in some ways they resemble aspergillosis in humans. Various causes of sea fan aspergillosis have been proposed: spores in dust clouds from the Sahara nutrient deposition from Sahara dust clouds, terrestrial runoff, and environmental stress. All published studies have focused on the fungus A. sydowii as the sole pathogen. However, preliminary data from Puerto Rico suggest that at least four species of Aspergillus infect sea fans, some of which are known to produce mycotoxins. The long-term goal of this project is to be able to predict and prevent aspergillosis in sea fans, and to understand common mechanisms of aspergillosis in human and sea fan infections. This project includes four key steps towards this goal: 1) determine which species of Aspergillus infect sea fans in Puerto Rico, by isolation of fungi and DNA sequencing; 2) determine if Aspergillus isolated from sea fans are pathogenic by fulfiilling Koch's postulates, using sea fans in aquarium tanks; 3) test the role of stress in susceptibility of sea fans to aspergillosis, by testing for presence of heatshock proteins; and 4) determine if the strains of Aspergillus isolated from sea fans produce mycotoxins in vitro, since mycotoxins contribute to pathogenicity in fungal infections of humans. The overall hypothesis is that various species of Aspergillus cause aspergillosis, that the pathogens are opportunistic, and that mycotoxins and environmental stress contribute to the disease. Understanding the causal agents, physiological responses and environmental factors that contribute to the disease are crucial steps towards prevention.
| Ramírez-Camejo, Luis A; Maldonado-Morales, Génesis; Bayman, Paul (2017) Differential Microbial Diversity in Drosophila melanogaster: Are Fruit Flies Potential Vectors of Opportunistic Pathogens? Int J Microbiol 2017:8526385 |
| Morales-Rivera, Keyla F; Piñero Cruz, Dalice M; Prieto, Jose A (2017) Crystal structure of (-)-(S)-4-[(2S,3S,4S,Z)-3-hydroxy-4-methyl-hept-5-en-2-yl]-1,3-dioxolan-2-one. Acta Crystallogr E Crystallogr Commun 73:1070-1072 |
| García-Arriaga, Marilyn; Acosta-Santiago, Maxier; Cruz, Antony et al. (2017) Probing the Limits of Supramolecular G-Quadruplexes Using Atomistic Molecular Dynamics Simulations. Inorganica Chim Acta 468:209-222 |
| Huang, Qing; Quiñones, Edwin (2016) A spectroscopic method to determine the activity of the restriction endonuclease EcoRV that involves a single reaction. Anal Biochem 497:103-5 |
| Mège, Pascal; Schizas, Nikolaos V; Reyes, Joselyd García et al. (2015) Genetic seascape of the threatened Caribbean elkhorn coral, Acropora palmata, on the Puerto Rico Shelf. Mar Ecol (Berl) 36:195-209 |
| Morales-Rivera, Amarilys; Hernández-Burgos, Mayté M; Martínez-Rivera, Arlene et al. (2014) Anxiolytic effects of oxytocin in cue-induced cocaine seeking behavior in rats. Psychopharmacology (Berl) 231:4145-55 |
| Ramírez-Camejo, Luis A; Torres-Ocampo, Ana P; Agosto-Rivera, José L et al. (2014) An opportunistic human pathogen on the fly: strains of Aspergillus flavus vary in virulence in Drosophila melanogaster. Med Mycol 52:211-9 |
| Galindo-Cardona, Alberto; Acevedo-Gonzalez, Jenny P; Rivera-Marchand, Bert et al. (2013) Genetic structure of the gentle Africanized honey bee population (gAHB) in Puerto Rico. BMC Genet 14:65 |
| Santiago-Rodriguez, Tasha M; Rivera, Jessica I; Coradin, Mariel et al. (2013) Antibiotic-resistance and virulence genes in Enterococcus isolated from tropical recreational waters. J Water Health 11:387-96 |
| Pantoja-Feliciano, Ida Gisela; Clemente, Jose C; Costello, Elizabeth K et al. (2013) Biphasic assembly of the murine intestinal microbiota during early development. ISME J 7:1112-5 |
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