Application): The overall objective of this proposal is to examine the hypothesis that expression of eicosanoid compounds in algae is related to stress conditions. The relationship is hypothesized to be based on eicosanoid action as an intracellular signal. Eicosanoids are biologically active molecules that are involved in many basic human metabolic functions, playing important roles in mammalian homeostasis where they are present in virtually every mammalian tissue and body fluid and where they exert a broad spectrum of physiological activities. Establishment that eicosanoids evolved as stress management compounds, and that their role has been conserved through evolution would provide a functional homology with one of its major roles in animals, where the lipoxygenase-mediated formation of leukotrienes and lipoxins serves as a signal to initiate stress responses within the organisms. The objectives will be carried out by culturing the target species, Murrayella periclados, and examining the time courses of lipoxygenase enzyme induction, eicosanoid expression, and subsequent stress protein expression. Stress protein identification will employ two-dimensional SDS PAGE. Eicosanoid concentrations will be measured using ELISA assays. A molecular probe for Murrayella lipoxygenase will be developed and the resultant gene or gene fragment will be utilized to produce polyclonal antibodies. The antibodies will then be used to follow lipoxygenase induction following stress conditions utilizing ELISA assays. That eicosanoids act as a signal will be tested with Murrayella that has been cultured to be deficient in lipoxygenase products.

Project Start
2002-05-01
Project End
2003-04-30
Budget Start
1997-10-01
Budget End
1998-09-30
Support Year
29
Fiscal Year
2002
Total Cost
$30,384
Indirect Cost
Name
University of Puerto Rico Mayaguez
Department
Type
DUNS #
City
Mayaguez
State
PR
Country
United States
Zip Code
00681
Díaz Casas, Adalberto; Chazin, Walter J; Pastrana-Ríos, Belinda (2017) Prp40 Homolog A Is a Novel Centrin Target. Biophys J 112:2529-2539
Lara Rodriguez, L; Sundaram, P A (2016) Corrosion behavior of plasma electrolytically oxidized gamma titanium aluminide alloy in simulated body fluid. Mater Chem Phys 181:67-77
Bueno-Vera, J A; Torres-Zapata, I; Sundaram, P A et al. (2015) Electrochemical characterization of MC3T3-E1 cells cultured on ?TiAl and Ti-6Al-4V alloys. Bioelectrochemistry 106:316-27
Pastrana-Rios, Belinda; Del Valle Sosa, Liliana; Santiago, Jorge (2015) Trifluoroacetic acid as excipient destabilizes melittin causing the selective aggregation of melittin within the centrin-melittin-trifluoroacetic acid complex. Struct Dyn 2:041711
Santiago-Medina, P; Sundaram, P A; Diffoot-Carlo, N (2015) Titanium Oxide: A Bioactive Factor in Osteoblast Differentiation. Int J Dent 2015:357653
Santiago-Medina, Pricilla; Sundaram, Paul A; Diffoot-Carlo, Nanette (2014) The effects of micro arc oxidation of gamma titanium aluminide surfaces on osteoblast adhesion and differentiation. J Mater Sci Mater Med 25:1577-87
Vera, José L; Rullán, Jorge; Santos, Natasha et al. (2014) Functionalized ferrocenes: The role of the para substituent on the phenoxy pendant group. J Organomet Chem 749:204-214
Lara Rodriguez, L; Sundaram, P A; Rosim-Fachini, E et al. (2014) Plasma electrolytic oxidation coatings on ?TiAl alloy for potential biomedical applications. J Biomed Mater Res B Appl Biomater 102:988-1001
Dominguez-Garcia, Moralba; Ortega-Zuniga, Carlos; Melendez, Enrique (2013) New tungstenocenes containing 3-hydroxy-4-pyrone ligands: antiproliferative activity on HT-29 and MCF-7 cell lines and binding to human serum albumin studied by fluorescence spectroscopy and molecular modeling methods. J Biol Inorg Chem 18:195-209
Pastrana-Rios, Belinda; Reyes, Myrna; De Orbeta, Jessica et al. (2013) Relative stability of human centrins and its relationship to calcium binding. Biochemistry 52:1236-48

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