This research focuses on the cellular and molecular processes that take place in hair cells of the sensory organs of the vestibular and auditory systems during development of the vertebrate ear. The aquatic amphibian, Xenopus laevis, will be used as a model system for the study of hair cells and their development. The broad objective of this research is to gain an integrated view of the development and proliferation of sensory hair cells of the internal ear, by using multidisciplinary approaches that draw on techniques from biophysics, anatomy, tissue culture, and molecular biology. Project goal are: (1) The patch clamp method will be used to determine the ionic mechanisms underlying signal transduction in Xenopus hair cells. Results of biophysical studies of hair cell electrical properties will be correlated with structural studies of hair cell morphology and stereociliary bundle characteristics during different stages of development. (2) Light, scanning, and transmission microscopy will be used to examine development of Xenopus hair cell structure and innervation. (3) Recombinant DNA technology will be used to clone genes for ion channels and cytoskeletal proteins expressed in Xenopus hair cells. (4) An in vitro culture preparation will be established and used for studies of hair cell regeneration. The knowledge gained from this research will advance understanding of normal vestibulo-auditory system function as well as dysfunction. Results will increase knowledge of signal transduction pathways in hair cells, and will reveal cellular and genetic events that underlie hair cell differentiation and development. An important long-term objective of this study is to understand the mechanisms that regulate hair cell proliferation and regeneration. The knowledge gained should prove useful in developing treatments for hearing disorders based on hair cell dysfunction, particularly those caused by trauma.

Project Start
1999-03-01
Project End
2000-02-29
Budget Start
1998-10-01
Budget End
1999-09-30
Support Year
25
Fiscal Year
1999
Total Cost
Indirect Cost
Name
New Mexico State University Las Cruces
Department
Type
DUNS #
City
Las Cruces
State
NM
Country
United States
Zip Code
88003
Pfister, Katherine; Shook, David R; Chang, Chenbei et al. (2016) Molecular model for force production and transmission during vertebrate gastrulation. Development 143:715-27
Edelaar, Pim; Roques, Severine; Hobson, Elizabeth A et al. (2015) Shared genetic diversity across the global invasive range of the monk parakeet suggests a common restricted geographic origin and the possibility of convergent selection. Mol Ecol 24:2164-76
Keyhaninejad, Neda; Curry, Jeanne; Romero, Joslynn et al. (2014) Fruit specific variability in capsaicinoid accumulation and transcription of structural and regulatory genes in Capsicum fruit. Plant Sci 215-216:59-68
Richins, Richard D; Kilcrease, James; Rodgriguez-Uribe, Laura et al. (2014) Carotenoid Extraction and Quantification from Capsicum annuum. Bio Protoc 4:
Unguez, Graciela A (2013) Electric fish: new insights into conserved processes of adult tissue regeneration. J Exp Biol 216:2478-86
Cuaron, Jesus A; Dulal, Santosh; Song, Yang et al. (2013) Tea tree oil-induced transcriptional alterations in Staphylococcus aureus. Phytother Res 27:390-6
Güth, Robert; Pinch, Matthew; Unguez, Graciela A (2013) Mechanisms of muscle gene regulation in the electric organ of Sternopygus macrurus. J Exp Biol 216:2469-77
Guerrero-Ferreira, Ricardo; Gorman, Clayton; Chavez, Alba A et al. (2013) Characterization of the bacterial diversity in Indo-West Pacific loliginid and sepiolid squid light organs. Microb Ecol 65:214-26
Young, Jennifer A; Karmakar, Sukhen; Jacobs, Hollie K et al. (2012) Synthetic approaches to mixed ligand chelators on t-butylphenol-formaldehyde oligomer (PFO) platforms. Tetrahedron 68:10030-10039
Ortega, Jose Luis; Wilson, Olivia L; Sengupta-Gopalan, Champa (2012) The 5' untranslated region of the soybean cytosolic glutamine synthetase ?(1) gene contains prokaryotic translation initiation signals and acts as a translational enhancer in plants. Mol Genet Genomics 287:881-93

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