We propose three sets of studies on experimental myopia: First, there is clear evidence from several laboratories that the eye enlargement and myopia produced by form deprivation in chicks is associated with increased growth of the posterior sclera. Furthermore, when form vision is restored to these animals the rate of scleral growth falls to less than normal, thereby reducing the relative elongation of the eye until it reattains normal shape and refraction. We propose in vitro studies utilizing this bidirectional modulation of growth to answer refraction. We propose in vitro studies utilizing this bidirectional modulation of growth to answer three questions: Is the altered scleral growth the consequence of the cells being continuously stimulated or inhibited or is it a consequence of a change in state of the cells? Can we show by conditioned medium experiments that the retina or other ocular tissues secrete factors that alter the growth of the scleral cells? Does the sclera secrete paracrine growth-modulating substances that differ depending on the visual circumstances? Second, we propose two questions to be addressed by in vivo studies of deprivation myopia: Because retinal dopamine levels are lower in chicks wearing diffusers, and dopamine agonists ameliorate deprivation myopia and eye elongation, do the agonists of dopamine act specifically to reduce the excessive growth associated with form deprivation or do they simply reduce eye growth in general? As the form-deprivation myopia shown in tree shrews is similar to that of chickens, is it also attributable to increased growth of the posterior sclera? Third, we propose several studies on the issue of to what extent can the eye use growth modulation to guide it toward emmetropia, if adequate vision is present. This situation has the advantage of being more similar to the one in which myopia develops in humans in that the eyes have unrestricted vision. Recently we have discovered a new process involved in emmetropization: The choroid can dramatically change its thickness by as much as 0.5mm and thereby compensate for refractive errors up to 8 diopters. We need to know what contribution to emmetropization is attributable to these choroidal changes and what is attributable to changes in growth of the globe (i.e. sclera).

Project Start
1997-02-01
Project End
1999-01-31
Budget Start
1997-10-01
Budget End
1998-09-30
Support Year
18
Fiscal Year
1998
Total Cost
Indirect Cost
Name
City College of New York
Department
Type
DUNS #
603503991
City
New York
State
NY
Country
United States
Zip Code
10031
Fried, Eric S; Li, Yue-Ming; Gilchrist, M Lane (2017) Phase Composition Control in Microsphere-Supported Biomembrane Systems. Langmuir 33:3028-3039
Beck, Cade; Singh, Tanya; Farooqi, Angela et al. (2016) Controlled microfluidics to examine growth-factor induced migration of neural progenitors in the Drosophila visual system. J Neurosci Methods 262:32-40
Gilchrist, M Lane; Ahn, Kwangwook; Li, Yue-Ming (2016) Imaging and Functional Analysis of ?-Secretase and Substrate in a Proteolipobead System with an Activity-Based Probe. Anal Chem 88:1303-11
Fried, Eric S; Luchan, Joshua; Gilchrist, M Lane (2016) Biodegradable, Tethered Lipid Bilayer-Microsphere Systems with Membrane-Integrated ?-Helical Peptide Anchors. Langmuir 32:3470-5
Banerjee, Shaibal; Sinha, Saikat; Pradhan, Padmanava et al. (2016) Regiospecifically Fluorinated Polycyclic Aromatic Hydrocarbons via Julia-Kocienski Olefination and Oxidative Photocyclization. Effect of Fluorine Atom Substitution on Molecular Shape. J Org Chem 81:3983-93
Salas-Ramirez, Kaliris Y; Bagnall, Ciara; Frias, Leslie et al. (2015) Doxorubicin and cyclophosphamide induce cognitive dysfunction and activate the ERK and AKT signaling pathways. Behav Brain Res 292:133-41
Thomson, Paul F; Parrish, Damon; Pradhan, Padmanava et al. (2015) Modular, Metal-Catalyzed Cycloisomerization Approach to Angularly Fused Polycyclic Aromatic Hydrocarbons and Their Oxidized Derivatives. J Org Chem 80:7435-46
Small, Chiyedza; Ramroop, Johnny; Otazo, Maria et al. (2014) An unexpected link between notch signaling and ROS in restricting the differentiation of hematopoietic progenitors in Drosophila. Genetics 197:471-83
Zhong, Lina; Tu, Raymond; Gilchrist, M Lane (2013) Tether-supported biomembranes with ?-helical peptide-based anchoring constructs. Langmuir 29:299-307
Guleyupoglu, Berkan; Schestatsky, Pedro; Edwards, Dylan et al. (2013) Classification of methods in transcranial electrical stimulation (tES) and evolving strategy from historical approaches to contemporary innovations. J Neurosci Methods 219:297-311

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