MutT/MutM/MutY Proteins and Gamma Ray Damage in E coli

Rodriguez, Adrian O.

Abstract

The major goal of this study is to characterize the effects of the radiation- induced oxidative DNA lesion, 7,8-dihydro-8-oxoguanine (8-OH-G) on mutagenesis and killing in the bacterium, Escherichia coli. We propose a genetic approach, using the well-characterized mutT, mutM and mutY strains that are defective in particular steps in the prevention of repair of 8-OH-G DNA lesions. The oxidative damage will be generated by ionizing radiation (137Cs gamma rays) under aerobic and anaerobic conditions. Radiation-induced 8-OH-G DNA lesions will be quantitated by ELISA assay and comparisons of radiation effects will be made with studies of H2O2-induced oxidative damage. A secondary goal of this study will be to determine the efficacy of a nitrone spin trap agent, such as N-tert-butyl-alpha-phenylnitrone (PBN), in reducing the lethal and mutagenic effects of radiation in wild-type and repair-defective E. coli strains. PBN react with the OH. radical which allows an evaluation which allows an evaluation of the role of OH. producing in producing 8-OH-G DNA lesions.

Funding Agency

• Agency: National Institute of Health (NIH)
• Institute: National Institute of General Medical Sciences (NIGMS)
• Type: Minority Biomedical Research Support - MBRS (S06)
• Project #: 5S06GM008192-22
• Application #: 6658415
• Study Section: Special Emphasis Panel (ZGM1)
• Project Start: 2002-09-01
• Project End: 2003-08-31
• Support Year: 22
• Fiscal Year: 2002

Institution

• Name: San Jose State University
• City: San Jose
• State: CA
• Country: United States
• Zip Code: 95112

Publications

Saleh, Nidal; Moore 2nd, Barry; Srebro, Monika et al. (2015) Acid/base-triggered switching of circularly polarized luminescence and electronic circular dichroism in organic and organometallic helicenes. Chemistry 21:1673-81

Saleh, Nidal; Srebro, Monika; Reynaldo, Thibault et al. (2015) enantio-Enriched CPL-active helicene-bipyridine-rhenium complexes. Chem Commun (Camb) 51:3754-7

Zhang, Xiao-Peng; Chang, Victoria Y; Liu, Jian et al. (2015) Potential switchable circularly polarized luminescence from chiral cyclometalated platinum(II) complexes. Inorg Chem 54:143-52

Sundar, M Shyam; Talele, Harish R; Mande, Hemant M et al. (2014) Synthesis of enantiomerically pure helicene like bis-oxazines from atropisomeric 7,7'-dihydroxy BINOL: Preliminary measurements of the circularly polarized luminescence. Tetrahedron Lett 55:1760-1764

Sánchez-Carnerero, Esther M; Moreno, Florencio; Maroto, Beatriz L et al. (2014) Circularly polarized luminescence by visible-light absorption in a chiral O-BODIPY dye: unprecedented design of CPL organic molecules from achiral chromophores. J Am Chem Soc 136:3346-9

Shen, Chengshuo; Anger, Emmanuel; Srebro, Monika et al. (2014) Straightforward access to mono- and bis-cycloplatinated helicenes that display circularly polarized phosphorescence using crystallization resolution methods. Chem Sci 5:1915-1927

Tohgha, Urice; Deol, Kirandeep K; Porter, Ashlin G et al. (2013) Ligand induced circular dichroism and circularly polarized luminescence in CdSe quantum dots. ACS Nano 7:11094-102

Brunet, Ernesto; Jimenez, Laura; de Victoria-Rodriguez, Maria et al. (2013) The use of lanthanide luminescence as a reporter in the solid state: Desymmetrization of the prochiral layers of ýý-zirconium phosphate/phosphonate and circularly polarized luminescence. Microporous Mesoporous Mater 169:222-234

Carey, Clayton M; Bueno, Raymund; Gutierrez, Daniel A et al. (2012) Recombinant rubistatin (r-Rub), an MVD disintegrin, inhibits cell migration and proliferation, and is a strong apoptotic inducer of the human melanoma cell line SK-Mel-28. Toxicon 59:241-8

Shirotani, Dai; Yamanari, Kazuaki; Kuroda, Reiko et al. (2012) Chiroptical spectra of tetrakis (+)-3-heptafluorobutylrylcamphorate Ln(III) complexes with an encapsulated alkali metal ion: solution structures as revealed by chiroptical spectra. Chirality 24:1055-62