This project proposes to examine the structures of low and high molecular weight mycelial glycopeptides from Penicillium charlesii and to determine whether these polymers serve as precursors and/or points of attachment or anchors for peptidophosphoglactomannans(PPGMS) or the extracellular glycohydrolases, phosphatases and proteases that are co-released with PPGMS. In order to establish the precursor/product relationships between the mycelial glycopeptides and the extracellular PPGMS, the structures of the cell wall, membrane, and extracellular PPGMS will be characterized. The structural relationships between the glycopeptides will be investigated by one and two dimensional NMR spectroscopy and GC/mass spectrometry in conjunction with amino acid analyses of peptide components. In addition, the biosynthetic relationships will be probed by using radiolabeled carbohydrate and peptide precursors an by biotinylation of cell walls, followed by isolation of the corresponding glycopeptides from the cell wall, membrane, and extracellular fractions. Establishing the anchor of these glycopeptides to the cell wall or membrane is important to the understanding of the physiological events leading to the release of PPGMS, hydrolytic enzymes, and potential allergens from the Penicillium charlesii.

Project Start
Project End
Budget Start
Budget End
Support Year
14
Fiscal Year
1995
Total Cost
Indirect Cost
Name
Colorado State University-Pueblo
Department
Type
DUNS #
City
Pueblo
State
CO
Country
United States
Zip Code
81001
Unis, Dave; Osborne, Cassandra; Diawara, Moussa M (2009) Arsenite exposure compromises early embryonic development in the Golden hamster. Reprod Toxicol 28:329-34
Kulkosky, Paul J; Wise, Valarie J; Brandt, Sara S et al. (2004) Interaction of TRH and CCK in the satiation of alcohol intake. Physiol Behav 82:53-6
Gonzales, D S; Bavister, B D; Mese, S A (2001) In utero and in vitro proteinase activity during the Mesocricetus auratus embryo zona escape time window. Biol Reprod 64:222-30
Kulkosky, P J; Allison, C T; Mattson, B J (2000) Thyrotropin releasing hormone decreases alcohol intake and preference in rats. Alcohol 20:87-91
Kulkosky, P J; Allison, C T; Allison, T G et al. (1998) Interaction of CCK and 8-OH-DPAT in the satiation of alcohol intake. Alcohol 16:305-9
Kulkosky, P J; Allison, T G; Carr, B A (1996) Angiotensin II reduces alcohol intake and choice in water- or food-restricted rats. Alcohol 13:359-63
Kulkosky, P J; Carr, B A; Flores, R K et al. (1995) Conditioned taste aversions induced by alcohol and lithium in rats selectively bred for ethanol neurosensitivity. Alcohol Clin Exp Res 19:945-50
Schnur, P; Espinoza, M; Flores, R (1994) Context-specific sensitization to naloxone-precipitated withdrawal in hamsters: effect of pimozide. Pharmacol Biochem Behav 48:791-7
Schnur, P; Espinoza, M; Flores, R (1992) Effects of diurnal phase and pimozide on cholecystokinin-elicited hypoactivity in the hamster. Pharmacol Biochem Behav 43:979-84
Schnur, P; Espinoza, M; Flores, R et al. (1992) Blocking naloxone-precipitated withdrawal in rats and hamsters. Pharmacol Biochem Behav 43:1093-8