The goal of this study is to improve the synthetic scope afforded by enzymatic catalysis in organic solvents, for asymmetric transformation leading to the synthesis of biomedical compounds. The mechanism of enzyme catalysis in non-aqueous media, and the effect or organic solvents on the enzyme structure is not well understood. As a result, the enzyme selectivity (pro-chiral selectivity and enantioslectivity) of any given reactions can not be predicted, and therefore, enzyme catalysis in organic solvents can not be used efficiently and successfully for the purposes mentioned above. The objectives of this study are: (a), to determine the mechanism of enzyme catalysis in organ solvents, by studying the effect of organic solvents on the structure of subtilisin C., and by studying the physicochemical properties of the solvents which affect the enzyme enantioselectivity; (b), to study the properties (enhanced enantioselectivity, stability, and catalytic rate)of subtilisin C.- cyclodextrin mixtures in organic solvents. This new enzyme preparation has been shown to exhibit higher reaction rates and enantioselectivity, and to preserve the enzyme secondary structure during lyophilization (preliminary results). Therefore, it has the potential to become the enzyme preparation of choice for the synthesis of biomedical relevant compounds. The ultimate goal of this project is to find a system in which the enzyme enantioslectivity of any given reaction can be rationally predicted in any solvent. Enzyme catalysis in organ solvents will become a powerful tool for the syntheses of chiral biologically active compounds.
| Gawronski, Katerina A B; Kim, Junhyong (2017) Single cell transcriptomics of noncoding RNAs and their cell-specificity. Wiley Interdiscip Rev RNA 8: |
| Falk, Samantha J; Lee, Jaehyoun; Sekulic, Nikolina et al. (2016) CENP-C directs a structural transition of CENP-A nucleosomes mainly through sliding of DNA gyres. Nat Struct Mol Biol 23:204-208 |
| Falk, Samantha J; Guo, Lucie Y; Sekulic, Nikolina et al. (2015) Chromosomes. CENP-C reshapes and stabilizes CENP-A nucleosomes at the centromere. Science 348:699-703 |
| Sanchez-Cruz, Pedro; Santos, Areli; Diaz, Stephany et al. (2014) Metal-independent reduction of hydrogen peroxide by semiquinones. Chem Res Toxicol 27:1380-6 |
| Piñero-Santiago, Luis E; García, Carmelo; Lhiaubet-Vallet, Virginie et al. (2013) Photooxidation mechanism of levomepromazine in different solvents. Photochem Photobiol 89:1479-89 |
| Bryant, Jessica M; Meyer-Ficca, Mirella L; Dang, Vanessa M et al. (2013) Separation of spermatogenic cell types using STA-PUT velocity sedimentation. J Vis Exp : |
| Castillo, Betzaida; Bromberg, Lev; López, Xaira et al. (2012) Intracellular Delivery of siRNA by Polycationic Superparamagnetic Nanoparticles. J Drug Deliv 2012:218940 |
| Sanchez-Cruz, Pedro; Dejesus-Andino, Francisco; Alegria, Antonio E (2012) Roles of hydrophilicities and hydrophobicities of dye and sacrificial electron donor on the photochemical pathway. J Photochem Photobiol A Chem 236:54-60 |
| Stepanenko, Viatcheslav; de Jesús, Melvin; Garcia, Carmelo et al. (2012) Synthesis and stability of new spiroaminoborate esters. Tetrahedron Lett 53:910-913 |
| Bansal, Vibha; Delgado, Yamixa; Legault, Marc et al. (2012) Low operational stability of enzymes in dry organic solvents: changes in the active site might affect catalysis. Molecules 17:1870-82 |
Showing the most recent 10 out of 65 publications
