Abstract

The goal of our program is to understand the function of two-component histidine kinases in the fungi Neurospora crassa and Candida albicans. Nik-1 and Cos1p are homologs and both are important during hyphal development in their respective organisms. We plan to define the other signaling proteins in each of the signal transduction pathways, analyze the biochemical properties of each protein, and determine the cell wall composition of wild type and knockout mutant organisms. As C. albicans is a pathogen, these studies should help define possible antifungal targets, as hyphal development is correlated with virulence in this organism. We will use N. crassa as a model for Cos1p function in C. albicans due to the ease of genetic manipulation in this organism. Nik-2 is another histidine kinase in N. crassa whose function is unknown and is not found in C. albicans. We will construct a null mutant and analyze the resultant phenotype. In addition we plan to define the other proteins in the Nik-2 signal transduction pathway and isolate the protein and analyze its biochemical proteins For all of the above proteins, we will also attempt to grow crystals with the hope of obtaining a three-dimensional structure.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Minority Biomedical Research Support - MBRS (S06)
Project #
5S06GM053933-05
Application #
6458441
Study Section
Minority Programs Review Committee (MPRC)
Project Start
2001-06-01
Project End
2002-05-31
Budget Start
Budget End
Support Year
5
Fiscal Year
2001
Total Cost
Indirect Cost

  Institution

Name
California State Polytechnic University Pomona
Department
Type
DUNS #
City
Pomona
State
CA
Country
United States
Zip Code
91768

  Publications

Dadgar, Saedeh; Floriano, Wely B (2015) Systematic discovery of molecular probes targeting multiple non-orthosteric and spatially distinct sites in the botulinum neurotoxin subtype A (BoNT/A). Mol Cell Probes 29:135-43
Dadgar, Saedeh; Ramjan, Zack; Floriano, Wely B (2013) Paclitaxel is an inhibitor and its boron dipyrromethene derivative is a fluorescent recognition agent for botulinum neurotoxin subtype A. J Med Chem 56:2791-803
Chew, Tina W; Jiang, Xinyin; Yan, Jian et al. (2011) Folate intake, MTHFR genotype, and sex modulate choline metabolism in mice. J Nutr 141:1475-81
Liang, M T C; Braun, W; Bassin, S L et al. (2011) Effect of high-impact aerobics and strength training on BMD in young women aged 20-35 years. Int J Sports Med 32:100-8
Yan, Jian; Wang, Wei; Gregory 3rd, Jesse F et al. (2011) MTHFR C677T genotype influences the isotopic enrichment of one-carbon metabolites in folate-compromised men consuming d9-choline. Am J Clin Nutr 93:348-55
Shin, William; Yan, Jian; Abratte, Christian M et al. (2010) Choline intake exceeding current dietary recommendations preserves markers of cellular methylation in a genetic subgroup of folate-compromised men. J Nutr 140:975-80
Austin, Misa U; Liau, Wei-Siang; Balamurugan, Krishnaswamy et al. (2010) Knockout of the folate transporter folt-1 causes germline and somatic defects in C. elegans. BMC Dev Biol 10:46
Caudill, Marie A; Dellschaft, Neele; Solis, Claudia et al. (2009) Choline intake, plasma riboflavin, and the phosphatidylethanolamine N-methyltransferase G5465A genotype predict plasma homocysteine in folate-deplete Mexican-American men with the methylenetetrahydrofolate reductase 677TT genotype. J Nutr 139:727-33
Ivanov, Alexandre; Nash-Barboza, Susan; Hinkis, Sabrina et al. (2009) Genetic variants in phosphatidylethanolamine N-methyltransferase and methylenetetrahydrofolate dehydrogenase influence biomarkers of choline metabolism when folate intake is restricted. J Am Diet Assoc 109:313-8
Lee, Justine; Bernard, Steven; Liu, Xiao-Chuan (2009) Nanostructured Biomimetic Catalysts for Asymmetric Hydrogenation of Enamides using Molecular Imprinting Technology. React Funct Polym 69:650-654

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