Abstract

The Limulus amoebocyte lysate (LAL) test reagent is used to make one h determinations of endotoxins in fresh ground meats. These values are then employed to calculate the total number of gram negative bacteria represented by the LAL-determined endotoxin. The estimated numbers of gram positive bacteria are determined by pre-determined ratios that exist in the fresh meats between gram negative bacteria and gram positives. Thus, the aerobic plate count of a fresh ground meat sample can be estimated in one h by use of LAL. Research during this phase of the overall project will focus on methods for extracting endotoxins from ground meats and their resolution by polyacrylamide gel methods. Initial studies on the effect of meat-borne microorganisms on endotoxins of meat origin will be initiated. Part 1 of 5.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Minority Biomedical Research Support - MBRS (S06)
Project #
5S06RR008167-09
Application #
3514086
Study Section
General Research Support Program Advisory Committee (GRS)
Project Start
1978-09-29
Project End
1987-12-31
Budget Start
1987-01-01
Budget End
1987-12-31
Support Year
9
Fiscal Year
1987
Total Cost
Indirect Cost

  Institution

Name
Wayne State University
Department
Type
Schools of Arts and Sciences
DUNS #
City
Detroit
State
MI
Country
United States
Zip Code
48202

  Publications

Yumoto, Chie; Jacobson, Sandra W; Jacobson, Joseph L (2008) Fetal substance exposure and cumulative environmental risk in an African American cohort. Child Dev 79:1761-76
Jacobson, Sandra W; Carr, Lucinda G; Croxford, Julie et al. (2006) Protective effects of the alcohol dehydrogenase-ADH1B allele in children exposed to alcohol during pregnancy. J Pediatr 148:30-7
Burden, Matthew J; Jacobson, Sandra W; Jacobson, Joseph L (2005) Relation of prenatal alcohol exposure to cognitive processing speed and efficiency in childhood. Alcohol Clin Exp Res 29:1473-83
Burden, Matthew J; Jacobson, Sandra W; Sokol, Robert J et al. (2005) Effects of prenatal alcohol exposure on attention and working memory at 7.5 years of age. Alcohol Clin Exp Res 29:443-52
Chiodo, Lisa M; Jacobson, Sandra W; Jacobson, Joseph L (2004) Neurodevelopmental effects of postnatal lead exposure at very low levels. Neurotoxicol Teratol 26:359-71
Jacobson, Sandra W; Jacobson, Joseph L; Sokol, Robert J et al. (2004) Maternal age, alcohol abuse history, and quality of parenting as moderators of the effects of prenatal alcohol exposure on 7.5-year intellectual function. Alcohol Clin Exp Res 28:1732-45
Jacobson, Sandra W; Chiodo, Lisa M; Sokol, Robert J et al. (2002) Validity of maternal report of prenatal alcohol, cocaine, and smoking in relation to neurobehavioral outcome. Pediatrics 109:815-25
Jacobson, S W; Chiodo, L M; Jacobson, J L (1999) Breastfeeding effects on intelligence quotient in 4- and 11-year-old children. Pediatrics 103:e71
Jacobson, S W; Jacobson, J L; Sokol, R J et al. (1996) New evidence for neurobehavioral effects of in utero cocaine exposure. J Pediatr 129:581-90
Jacobson, J L; Jacobson, S W (1996) Prospective, longitudinal assessment of developmental neurotoxicity. Environ Health Perspect 104 Suppl 2:275-83

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