Abstract

(must be no longer than 30 lines of text) Cryo-EM is a powerful tool for visualizing molecular and cellular structures. Stony Brook University is one of the 62 research universities that comprise the Association of American Universities (AAU), an invitation-only organization of leading research universities in North America. There are a large number of biomedical researchers on campus. Many NIH-funded projects will benefit from having access to a cryo-EM facility. There will be two cryo-EM faculty members including the applicant who has been at the university since 2010, but there is no cryo-EM facility on campus. We therefore request a 200-KV cryo-TEM equipped with a direct detection camera. The new instrument is compatible with several existing automation software. Thus acquisition of the new microscope will enable cryo-EM research, increase productivity, and satisfy the need of this large life science community.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
Office of The Director, National Institutes of Health (OD)
Type
Biomedical Research Support Shared Instrumentation Grants (S10)
Project #
1S10OD012272-01A1
Application #
8730316
Study Section
Special Emphasis Panel (ZRG1)
Program Officer
Levy, Abraham
Project Start
2015-07-01
Project End
2017-06-30
Budget Start
2015-07-01
Budget End
2017-06-30
Support Year
1
Fiscal Year
2015
Total Cost
Indirect Cost

  Institution

Name
State University New York Stony Brook
Department
Type
University-Wide
DUNS #
804878247
City
Stony Brook
State
NY
Country
United States
Zip Code
11794

  Publications

Yuan, Zuanning; Riera, Alberto; Bai, Lin et al. (2017) Structural basis of Mcm2-7 replicative helicase loading by ORC-Cdc6 and Cdt1. Nat Struct Mol Biol 24:316-324
Yuan, Zuanning; Bai, Lin; Sun, Jingchuan et al. (2016) Structure of the eukaryotic replicative CMG helicase suggests a pumpjack motion for translocation. Nat Struct Mol Biol 23:217-24
O'Donnell, Mike; Li, Huilin (2016) The Eukaryotic Replisome Goes Under the Microscope. Curr Biol 26:R247-56
Jastrab, Jordan B; Wang, Tong; Murphy, J Patrick et al. (2015) An adenosine triphosphate-independent proteasome activator contributes to the virulence of Mycobacterium tuberculosis. Proc Natl Acad Sci U S A 112:E1763-72
Sun, Jingchuan; Fernandez-Cid, Alejandra; Riera, Alberto et al. (2014) Structural and mechanistic insights into Mcm2-7 double-hexamer assembly and function. Genes Dev 28:2291-303
Sun, Jingchuan; Evrin, Cecile; Samel, Stefan A et al. (2013) Cryo-EM structure of a helicase loading intermediate containing ORC-Cdc6-Cdt1-MCM2-7 bound to DNA. Nat Struct Mol Biol 20:944-51