Abstract

This is an application requesting funds to purchase a Q Exactive mass spectrometer and liquid chromatography system that will be used for quantitative proteomics research. The instrument will be integrated into the Robert Wood Johnson Medical School/Rutgers University Biological Mass Spectrometry Facility. Current instrumentation in the Facility is used at maximal capacity and we require additional capabilities to conduct quantitative proteomics experiments for thirteen NIH-funded investigators. Our user group will apply quantitative mass spectrometry to most efficiently address a wide range of important biomedical research areas including neurological disease, bacterial and viral infection, cancer, anaphylaxis, diabetes, digestive disease, and sleep disorders. Specific experiments include quantification of changes in levels and post-translational modifications of clock proteins throughout the circadian cycle, a systems biology approach to understand the lysosome and its role in human neurodegenerative disease, persistence of epitopes on protein food allergens under conditions that simulate gastric digestion, dynamics of post- translational modification of tumor suppressors during oncogenesis and analysis of pathogen-host interactions at different stages in their life cycle for hepatitis C virus, retroviruses, and chlamydia.. The requested instrument will therefore represent a significant asset to our research community and will accelerate the pace of basic and applied NIH-funded health-research at Rutgers University and the Robert Wood Johnson Medical School of UMDNJ.

Public Health Relevance

This is a proposal requesting funds to acquire a specialized mass spectrometer that will be used for projects being conducted by thirteen NIH-supported investigators. The availability of this instrument will accelerate the pace of basic and applied biomedical research into important areas including neurological disease, bacterial and viral infection, sleep disorders, diabetes, food allergies and cancer.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
Office of The Director, National Institutes of Health (OD)
Type
Biomedical Research Support Shared Instrumentation Grants (S10)
Project #
1S10OD016400-01A1
Application #
8640415
Study Section
Special Emphasis Panel (ZRG1)
Program Officer
Birken, Steven
Project Start
2014-07-01
Project End
2015-06-30
Budget Start
2014-07-01
Budget End
2015-06-30
Support Year
1
Fiscal Year
2014
Total Cost
Indirect Cost

  Institution

Name
Rbhs-Robert Wood Johnson Medical School
Department
Type
Schools of Medicine
DUNS #
City
Piscataway
State
NJ
Country
United States
Zip Code
08854

  Publications

Poillet-Perez, Laura; Xie, Xiaoqi; Zhan, Le et al. (2018) Autophagy maintains tumour growth through circulating arginine. Nature 563:569-573
Solé-Domènech, Santiago; Rojas, Ana V; Maisuradze, Gia G et al. (2018) Lysosomal enzyme tripeptidyl peptidase 1 destabilizes fibrillar A? by multiple endoproteolytic cleavages within the ?-sheet domain. Proc Natl Acad Sci U S A 115:1493-1498
Her, Joonyoung; Ray, Chandni; Altshuler, Jake et al. (2018) 53BP1 Mediates ATR-Chk1 Signaling and Protects Replication Forks under Conditions of Replication Stress. Mol Cell Biol 38:
Liu, Juan; Zhang, Cen; Zhao, Yuhan et al. (2017) Parkin targets HIF-1? for ubiquitination and degradation to inhibit breast tumor progression. Nat Commun 8:1823
Yue, Xuetian; Zhang, Cen; Zhao, Yuhan et al. (2017) Gain-of-function mutant p53 activates small GTPase Rac1 through SUMOylation to promote tumor progression. Genes Dev 31:1641-1654
Cai, Na; Bai, Zhiyong; Nanda, Vikas et al. (2017) Mass Spectrometric Analysis of TRPM6 and TRPM7 Phosphorylation Reveals Regulatory Mechanisms of the Channel-Kinases. Sci Rep 7:42739
Denzin, Lisa K; Khan, Aly A; Virdis, Francesca et al. (2017) Neutralizing Antibody Responses to Viral Infections Are Linked to the Non-classical MHC Class II Gene H2-Ob. Immunity 47:310-322.e7
Mehta, Anand; Comunale, Mary Ann; Rawat, Siddhartha et al. (2016) Intrinsic hepatocyte dedifferentiation is accompanied by upregulation of mesenchymal markers, protein sialylation and core alpha 1,6 linked fucosylation. Sci Rep 6:27965
Wang, Mengjun; Comunale, Mary Ann; Herrera, Harmin et al. (2016) Identification of IgM as a contaminant in lectin-FLISA assays for HCC detection. Biochem Biophys Res Commun 476:140-5
Yue, Xuetian; Zhao, Yuhan; Huang, Grace et al. (2016) A novel mutant p53 binding partner BAG5 stabilizes mutant p53 and promotes mutant p53 GOFs in tumorigenesis. Cell Discov 2:16039

Showing the most recent 10 out of 23 publications