Abstract

This proposal requests funds for the purchase of a plasma desorption mass spectrometer (PDMS) with time-of-flight (TOF) analytical capability. It is planned that the instrument will serve primarily 12 user groups in 10 departments at the University of Washington. Virtually al of the projects are directed at analysis of peptides of biomedical interest. This instrumentation will provide these users with access for the first time to mass spectrometer analyses of peptides of mass greater than 3000 amu. This biomedical community will benefit from access to this rapid, sensitive, flexible technique for examining peptides of high mass, for detecting posttranslational modifications, and for application of chemical strategies of partial structural analysis of proteins.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Biomedical Research Support Shared Instrumentation Grants (S10)
Project #
1S10RR005543-01
Application #
3520535
Study Section
Special Emphasis Panel (SSS (B))
Project Start
1990-05-24
Project End
1991-05-23
Budget Start
1990-05-24
Budget End
1991-05-23
Support Year
1
Fiscal Year
1990
Total Cost
Indirect Cost

  Institution

Name
University of Washington
Department
Type
Schools of Medicine
DUNS #
135646524
City
Seattle
State
WA
Country
United States
Zip Code
98195

  Publications

Resing, K A; Thulin, C; Whiting, K et al. (1995) Characterization of profilaggrin endoproteinase 1. A regulated cytoplasmic endoproteinase of epidermis. J Biol Chem 270:28193-8
Resing, K A; Johnson, R S; Walsh, K A (1995) Mass spectrometric analysis of 21 phosphorylation sites in the internal repeat of rat profilaggrin, precursor of an intermediate filament associated protein. Biochemistry 34:9477-87
Resing, K A; Mansour, S J; Hermann, A S et al. (1995) Determination of v-Mos-catalyzed phosphorylation sites and autophosphorylation sites on MAP kinase kinase by ESI/MS. Biochemistry 34:2610-20
Berger, S J; Resing, K A; Taylor, T C et al. (1995) Mass-spectrometric analysis of ADP-ribosylation factors from bovine brain: identification and evidence for homogeneous acylation with the C14:0 fatty acid (myristate). Biochem J 311 ( Pt 1):125-32
Jin, L; Davis, M R; Hu, P et al. (1994) Identification of novel glutathione conjugates of disulfiram and diethyldithiocarbamate in rat bile by liquid chromatography-tandem mass spectrometry. Evidence for metabolic activation of disulfiram in vivo. Chem Res Toxicol 7:526-33
Resing, K A; Johnson, R S; Walsh, K A (1993) Characterization of protease processing sites during conversion of rat profilaggrin to filaggrin. Biochemistry 32:10036-45
Baillie, T A; Davis, M R (1993) Mass spectrometry in the analysis of glutathione conjugates. Biol Mass Spectrom 22:319-25
Davis, M R; Kassahun, K; Jochheim, C M et al. (1993) Glutathione and N-acetylcysteine conjugates of 2-chloroethyl isocyanate. Identification as metabolites of N,N'-bis(2-chloroethyl)-N-nitrosourea in the rat and inhibitory properties toward glutathione reductase in vitro. Chem Res Toxicol 6:376-83
Hanson, D A; Weis, M A; Bollen, A M et al. (1992) A specific immunoassay for monitoring human bone resorption: quantitation of type I collagen cross-linked N-telopeptides in urine. J Bone Miner Res 7:1251-8
Johnson, R S; Walsh, K A (1992) Sequence analysis of peptide mixtures by automated integration of Edman and mass spectrometric data. Protein Sci 1:1083-91

Showing the most recent 10 out of 12 publications